Recombinant Acidaminococcus sp. CRISPR-associated endonuclease Cas12a (cas12a), Acidaminococcus sp. (strain BV3L6), E.coli, N-terminal 10xHis-tagged and C-terminal Myc-tagged, 158.6kDa, purity >90% as determined by SDS-PAGE.

Recombinant Acidaminococcus sp. CRISPR-associated endonuclease Cas12a (cas12a)

Recombinant Acidaminococcus sp. CRISPR-associated endonuclease Cas12a (cas12a) — CRISPR (clustered regularly interspaced short palindromic repeat), is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugat… Purity >90%.

SKU: BCRECP-002292 Category:
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Product Specifications

Product SkuBCRECP-002292
Product DescriptionRecombinant Acidaminococcus sp. CRISPR-associated endonuclease Cas12a (cas12a) Protein is expressed from E.coli with N-terminal 10xHis-tagged and C-terminal Myc-tagged. It contains 1-1307aa. [Accession | U2UMQ6].
Uniprot No.U2UMQ6
Gene Namescas12a
PurityGreater than 90% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region1-1307aa
SpeciesAcidaminococcus sp. (strain BV3L6)
Tag InfoN-terminal 10xHis-tagged and C-terminal Myc-tagged
Molecular weight158.6kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasOthers

Function

CRISPR (clustered regularly interspaced short palindromic repeat), is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain sequences complementary to antecedent mobile elements and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA).

Product Specifications

Recombinant Acidaminococcus sp. CRISPR-associated endonuclease Cas12a (cas12a) is a recombinant protein from Acidaminococcus sp. (strain BV3L6), expressed in E.coli, covering amino acids 1-1307aa, with N-terminal 10xHis-tagged and C-terminal Myc-tagged tag, molecular weight 158.6kDa, purity Greater than 90% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

Recommended applications depend on the target, product format, and product-specific validation data. Refer to the product datasheet or Certificate of Analysis (CoA), or contact BioCrest Sci for application guidance.

Biological Activity: Please contact us to obtain bioactivity data.

Storage: Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shipping & Lead Time: Availability, shipping conditions, and estimated lead time are confirmed in the formal quotation or order confirmation based on the product format, destination, and order requirements.

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Protein Biology

Function

CRISPR (clustered regularly interspaced short palindromic repeat), is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain sequences complementary to antecedent mobile elements and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA). Recognizes a short motif in the CRISPR repeat sequences (the 5' PAM or protospacer adjacent motif, TTTN in this organism) to help distinguish self versus nonself, as targets within the bacterial CRISPR locus do not have PAMs (PubMed:26422227). Has dsDNA endonuclease activity, results in staggered 4-base 5' overhangs 19 and 22 bases downstream of the PAM on the non-targeted and targeted strand respectively (PubMed:26422227). Non-target strand cleavage by the RuvC domain is probably a prerequisite of target strand cleavage by the Nuc domain (PubMed:27114038). Protects E.coli against plasmids and bacteriophage M13mp18, phage T4 with hydroxymethyl or unmodified (but not glycosylated) cytosines and to a lesser extent against lambda and VpaE1 phage (PubMed:38261981). In this CRISPR system correct processing of pre-crRNA requires only this protein and the CRISPR locus (By similarity)

Subunit

Monomer (PubMed:27114038)

Gene: cas12a  |  Organism: Acidaminococcus sp. (strain BV3L6)  |  Synonyms: AsCpf1; CRISPR-associated endonuclease Cpf1
Key Publications

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Submit the Online Inquiry form with your required quantity and specifications, or email sales@biocrestsci.com. Available sizes, pricing, stock status, documentation, and estimated lead time are confirmed in the formal quotation.

What is the shipping and delivery time?

Availability, dispatch timing, shipping conditions, and delivery estimates depend on the product format, destination, and order requirements. These details are confirmed in the formal quotation or order confirmation.

How should this product be stored?

Follow the storage and handling instructions in the product-specific datasheet and Certificate of Analysis (CoA). Requirements may vary by product form and formulation; contact BioCrest Sci if product-specific guidance is needed.

What quality controls are performed on your products?

Quality-control data such as identity, purity, endotoxin level, or biological activity are provided only when specified on this product page, the datasheet, or Certificate of Analysis (CoA). Biological activity is not implied unless explicitly stated.

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