Recombinant Adeno-associated virus 2 Protein Rep78 (Rep78), partial, Adeno-associated virus 2 (isolate Srivastava/1982) (AAV-2), E.coli, C-terminal 6xHis-tagged, 31.2kDa, purity >90% as determined by SDS-PAGE.
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Recombinant Adeno-associated virus 2 Protein Rep78 (Rep78), partial

Recombinant Adeno-associated virus 2 Protein Rep78 (Rep78), partial — Plays an essential role in the initiation of viral DNA synthesis. Purity >90%.

SKU: BCRECP-004259 Categories: ,
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Product Specifications

Product SkuBCRECP-004259
Product DescriptionRecombinant Adeno-associated virus 2 Protein Rep78 (Rep78) Protein is expressed from E.coli with C-terminal 6xHis-tagged. It contains 1-210aa. [Accession | Q89268].
Uniprot No.Q89268
Gene NamesRep78
PurityGreater than 90% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region1-210aa
SpeciesAdeno-associated virus 2 (isolate Srivastava/1982) (AAV-2)
Tag InfoC-terminal 6xHis-tagged
Molecular weight31.2kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasOthers

Function

Plays an essential role in the initiation of viral DNA synthesis. Binds specifically to an inverted terminal repeat element (ITR) on the 3' and 5' ends of the viral DNA, where it cleaves a site specifically to generate a priming site for initiation of the synthesis of a complementary strand. Also plays a role as transcriptional regulator, DNA helicase and as key factors in site-specific integration of the viral genome.

Biological Context

Subcellular Location: Host nucleus

Product Specifications

Recombinant Adeno-associated virus 2 Protein Rep78 (Rep78), partial is a recombinant protein from Adeno-associated virus 2 (isolate Srivastava/1982) (AAV-2), expressed in E.coli, covering amino acids 1-210aa, with C-terminal 6xHis-tagged tag, molecular weight 31.2kDa, purity Greater than 90% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

Recommended applications depend on the target, product format, and product-specific validation data. Refer to the product datasheet or Certificate of Analysis (CoA), or contact BioCrest Sci for application guidance.

Biological Activity: Please contact us to obtain bioactivity data.

Storage: Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shipping & Lead Time: Availability, shipping conditions, and estimated lead time are confirmed in the formal quotation or order confirmation based on the product format, destination, and order requirements.

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Protein Biology

Function

Plays an essential role in the initiation of viral DNA synthesis. Binds specifically to an inverted terminal repeat element (ITR) on the 3' and 5' ends of the viral DNA, where it cleaves a site specifically to generate a priming site for initiation of the synthesis of a complementary strand. Also plays a role as transcriptional regulator, DNA helicase and as key factors in site-specific integration of the viral genome. Regulates host PKA activity by interacting with host PRKX as a mechanism of interfering with helper virus propagation and promoting its own replication. Inhibits the host cell cycle G1/S, S and G2/M transitions. These arrests may provide essential cellular factors for viral DNA replication

Subcellular Location

Host nucleus

Subunit

Hexamer when associated with the viral DNA ori sequence. Interacts with host PRKX. Interacts with host TOPORS. Interacts with host TBP and SUB1/PC4; these interactions play important roles in transcriptional regulation

Gene: Rep78  |  Organism: Adeno-associated virus 2 (isolate Srivastava/1982)
Key Publications

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How do I order or inquire about this product?

Submit the Online Inquiry form with your required quantity and specifications, or email sales@biocrestsci.com. Available sizes, pricing, stock status, documentation, and estimated lead time are confirmed in the formal quotation.

What is the shipping and delivery time?

Availability, dispatch timing, shipping conditions, and delivery estimates depend on the product format, destination, and order requirements. These details are confirmed in the formal quotation or order confirmation.

How should this product be stored?

Follow the storage and handling instructions in the product-specific datasheet and Certificate of Analysis (CoA). Requirements may vary by product form and formulation; contact BioCrest Sci if product-specific guidance is needed.

What quality controls are performed on your products?

Quality-control data such as identity, purity, endotoxin level, or biological activity are provided only when specified on this product page, the datasheet, or Certificate of Analysis (CoA). Biological activity is not implied unless explicitly stated.

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