Recombinant Amanita muscaria DOPA 4,5-dioxygenase (DODA), Amanita muscaria (Fly agaric), E.coli, N-terminal 10xHis-tagged and C-terminal Myc-tagged, 33.6kDa, purity >90% as determined by SDS-PAGE.

Recombinant Amanita muscaria DOPA 4,5-dioxygenase (DODA)

Recombinant Amanita muscaria DOPA 4,5-dioxygenase (DODA) — Extradiol dioxygenase that opens up the cyclic ring of DOPA between carbons 4 and 5 thus producing an unstable seco-DOPA that rearranges non-enzymatically to betalamic acid. Purity >90%.

SKU: BCRECP-002551 Category:
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Product Specifications

Product SkuBCRECP-002551
Product DescriptionRecombinant Amanita muscaria DOPA 4,5-dioxygenase (DODA) Protein is expressed from E.coli with N-terminal 10xHis-tagged and C-terminal Myc-tagged. It contains 1-228aa. [Accession | P87064].
Uniprot No.P87064
Gene NamesDODA
PurityGreater than 90% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region1-228aa
SpeciesAmanita muscaria (Fly agaric)
Tag InfoN-terminal 10xHis-tagged and C-terminal Myc-tagged
Molecular weight33.6kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasNeuroscience

Function

Extradiol dioxygenase that opens up the cyclic ring of DOPA between carbons 4 and 5 thus producing an unstable seco-DOPA that rearranges non-enzymatically to betalamic acid. Can also catalyze the formation of muscaflavin (a pigment found in the hygrocybe mushrooms family and of some amanita species only) by a 2,3-extradiol cleavage of DOPA.

Product Specifications

Recombinant Amanita muscaria DOPA 4,5-dioxygenase (DODA) — Extradiol dioxygenase that opens up the cyclic ring of DOPA between carbons 4 and 5 thus producing an unstable seco-DOPA that rearranges non-enzymatically to betalamic acid. Purity >90%. Suitable for ELISA and Western Blot applications.

Recommended applications depend on the target, product format, and product-specific validation data. Refer to the product datasheet or Certificate of Analysis (CoA), or contact BioCrest Sci for application guidance.

Biological Activity: Please contact us to obtain bioactivity data.

Storage: Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shipping & Lead Time: Availability, shipping conditions, and estimated lead time are confirmed in the formal quotation or order confirmation based on the product format, destination, and order requirements.

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Protein Biology

Function

Extradiol dioxygenase that opens up the cyclic ring of DOPA between carbons 4 and 5 thus producing an unstable seco-DOPA that rearranges non-enzymatically to betalamic acid. Can also catalyze the formation of muscaflavin (a pigment found in the hygrocybe mushrooms family and of some amanita species only) by a 2,3-extradiol cleavage of DOPA

Subcellular Location

Cytoplasm

Tissue Specificity

Expressed at high level in coloured cap tissue and at least 10 times lower level in the stipe

Subunit

Homodimer

Pathway

Pigment biosynthesis; betalain biosynthesis

Gene: DODA  |  Organism: Amanita muscaria

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Submit the Online Inquiry form with your required quantity and specifications, or email sales@biocrestsci.com. Available sizes, pricing, stock status, documentation, and estimated lead time are confirmed in the formal quotation.

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Availability, dispatch timing, shipping conditions, and delivery estimates depend on the product format, destination, and order requirements. These details are confirmed in the formal quotation or order confirmation.

How should this product be stored?

Follow the storage and handling instructions in the product-specific datasheet and Certificate of Analysis (CoA). Requirements may vary by product form and formulation; contact BioCrest Sci if product-specific guidance is needed.

What quality controls are performed on your products?

Quality-control data such as identity, purity, endotoxin level, or biological activity are provided only when specified on this product page, the datasheet, or Certificate of Analysis (CoA). Biological activity is not implied unless explicitly stated.

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