Recombinant Anemonia sulcata Delta-actitoxin-Avd1c, Anemonia sulcata (Mediterranean snakelocks sea anemone), Yeast, N-terminal 6xHis-tagged, 6.9kDa, purity >90% as determined by SDS-PAGE.

Recombinant Anemonia sulcata Delta-actitoxin-Avd1c

Recombinant Anemonia sulcata Delta-actitoxin-Avd1c — Binds specifically to voltage-gated sodium channels (Nav) (site 3), thereby delaying their inactivation. Purity >90%.

SKU: BCRECP-003814 Category:
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Product Specifications

Product SkuBCRECP-003814
Product DescriptionRecombinant Anemonia sulcata Delta-actitoxin-Avd1c Protein is expressed from Yeast with N-terminal 6xHis-tagged. It contains 1-47aa. [Accession | P01528].
Uniprot No.P01528
PurityGreater than 90% as determined by SDS-PAGE.
Expression SystemYeast
Expression Region1-47aa
SpeciesAnemonia sulcata (Mediterranean snakelocks sea anemone)
Tag InfoN-terminal 6xHis-tagged
Molecular weight6.9kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasOthers

Function

Binds specifically to voltage-gated sodium channels (Nav) (site 3), thereby delaying their inactivation. Has a strong effect on crustaceans and insects (DmNav1) and a weaker effect on mammals. This toxin is highly potent at mammalian Nav1.1/SCN1A (EC(50)=6.01 nM) and Nav1.2/SCN2A (EC(50)=7.88 nM).

Biological Context

Subcellular Location: Secreted; Nematocyst

Product Specifications

Recombinant Anemonia sulcata Delta-actitoxin-Avd1c is a recombinant protein from Anemonia sulcata (Mediterranean snakelocks sea anemone), expressed in Yeast, covering amino acids 1-47aa, with N-terminal 6xHis-tagged tag, molecular weight 6.9kDa, purity Greater than 90% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

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Protein Biology

Function

Binds specifically to voltage-gated sodium channels (Nav) (site 3), thereby delaying their inactivation. Has a strong effect on crustaceans and insects (DmNav1) and a weaker effect on mammals. This toxin is highly potent at mammalian Nav1.1/SCN1A (EC(50)=6.01 nM) and Nav1.2/SCN2A (EC(50)=7.88 nM) (PubMed:15169781). It also has great activity on Nav1.5/SCN5A (EC(50)=49.05 nM), Nav1.4/SCN4A (EC(50)=109.49 nM) and Nav1.6/SCN8A (EC(50)=about 180 nM) and is less potent on Nav1.3/SCN3A (EC(50)=759.22 nM) (when measured as the increase in the slow component) (PubMed:15169781)

Subcellular Location

Secreted; Nematocyst

Organism: Anemonia sulcata  |  Synonyms: ATX-II; Anemonia sulcata toxin 2; Neurotoxin 2; Toxin II
Key Publications

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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