Recombinant Aquifex aeolicus Reverse gyrase 2 (rgy2), partial, Aquifex aeolicus (strain VF5), E.coli, N-terminal 10xHis-tagged and C-terminal Myc-tagged, 39.5kDa, purity >90% as determined by SDS-PAGE.

Recombinant Aquifex aeolicus Reverse gyrase 2 (rgy2), partial

Recombinant Aquifex aeolicus Reverse gyrase 2 (rgy2), partial — Modifies the topological state of DNA by introducing positive supercoils in an ATP-dependent process, increasing the linking number in steps of +1. Purity >90%.

SKU: BCRECP-004270 Category:
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Product Specifications

Product SkuBCRECP-004270
Product DescriptionRecombinant Aquifex aeolicus Reverse gyrase 2 (rgy2) Protein is expressed from E.coli with N-terminal 10xHis-tagged and C-terminal Myc-tagged. It contains 1-280aa. [Accession | O67226].
Uniprot No.O67226
Gene Namesrgy2
PurityGreater than 90% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region1-280aa
SpeciesAquifex aeolicus (strain VF5)
Tag InfoN-terminal 10xHis-tagged and C-terminal Myc-tagged
Molecular weight39.5kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasOthers

Function

Modifies the topological state of DNA by introducing positive supercoils in an ATP-dependent process, increasing the linking number in steps of +1. Binds to single-stranded DNA, transiently cleaves and then rejoins the ends, introducing a positive supercoil in the process. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate.

Biological Context

Subcellular Location: Cytoplasm

Product Specifications

Recombinant Aquifex aeolicus Reverse gyrase 2 (rgy2), partial is a recombinant protein from Aquifex aeolicus (strain VF5), expressed in E.coli, covering amino acids 1-280aa, with N-terminal 10xHis-tagged and C-terminal Myc-tagged tag, molecular weight 39.5kDa, purity Greater than 90% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

Recommended applications depend on the target, product format, and product-specific validation data. Refer to the product datasheet or Certificate of Analysis (CoA), or contact BioCrest Sci for application guidance.

Biological Activity: Please contact us to obtain bioactivity data.

Storage: Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shipping & Lead Time: Availability, shipping conditions, and estimated lead time are confirmed in the formal quotation or order confirmation based on the product format, destination, and order requirements.

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Protein Biology

Function

Modifies the topological state of DNA by introducing positive supercoils in an ATP-dependent process, increasing the linking number in steps of +1. Binds to single-stranded DNA, transiently cleaves and then rejoins the ends, introducing a positive supercoil in the process. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate. Probably involved in rewinding DNA strands in regions of the chromosome that have opened up to allow replication, transcription, DNA repair and/or for DNA protection

Subcellular Location

Cytoplasm

Subunit

Monomer

Gene: rgy2  |  Organism: Aquifex aeolicus (strain VF5)

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Follow the storage and handling instructions in the product-specific datasheet and Certificate of Analysis (CoA). Requirements may vary by product form and formulation; contact BioCrest Sci if product-specific guidance is needed.

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Quality-control data such as identity, purity, endotoxin level, or biological activity are provided only when specified on this product page, the datasheet, or Certificate of Analysis (CoA). Biological activity is not implied unless explicitly stated.

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