Recombinant Arabidopsis thaliana Alpha-1,3-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase (GNTI), partial, Arabidopsis thaliana (Mouse-ear cress), Baculovirus, C-terminal 6xHis-tagged, 51.6kDa, purity >90% as determined by SDS-PAGE.
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Recombinant Arabidopsis thaliana Alpha-1,3-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase (GNTI), partial

Recombinant Arabidopsis thaliana Alpha-1,3-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase (GNTI), partial — Initiates complex N-linked carbohydrate formation. Purity >90%.

SKU: BCRECP-002883 Categories: ,
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Product Specifications

Product SkuBCRECP-002883
Product DescriptionRecombinant Arabidopsis thaliana Alpha-1,3-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase (GNTI) Protein is expressed from Baculovirus with C-terminal 6xHis-tagged. It contains 25-444aa. [Accession | Q9XGM8].
Uniprot No.Q9XGM8
Gene NamesGNTI
PurityGreater than 90% as determined by SDS-PAGE.
Expression SystemBaculovirus
Expression Region25-444aa
SpeciesArabidopsis thaliana (Mouse-ear cress)
Tag InfoC-terminal 6xHis-tagged
Molecular weight51.6kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasSignal Transduction

Function

Initiates complex N-linked carbohydrate formation. Essential for the conversion of high-mannose to hybrid and complex N-glycans. Required for normal root growth and morphology.

Biological Context

Subcellular Location: Golgi apparatus membrane (Single-pass type II membrane protein)
Tissue Specificity: Expressed in roots, stems, leaves and flowers
Pathway: Protein modification; protein glycosylation

Product Specifications

Recombinant Arabidopsis thaliana Alpha-1,3-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase (GNTI), partial is a recombinant protein from Arabidopsis thaliana (Mouse-ear cress), expressed in Baculovirus, covering amino acids 25-444aa, with C-terminal 6xHis-tagged tag, molecular weight 51.6kDa, purity Greater than 90% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

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Protein Biology

Function

Initiates complex N-linked carbohydrate formation. Essential for the conversion of high-mannose to hybrid and complex N-glycans. Required for normal root growth and morphology

Subcellular Location

Golgi apparatus membrane (Single-pass type II membrane protein)

Tissue Specificity

Expressed in roots, stems, leaves and flowers

Pathway

Protein modification; protein glycosylation

Gene: GNTI  |  Organism: Arabidopsis thaliana  |  Synonyms: N-acetylglucosaminyltransferase I; N-glycosyl-oligosaccharide-glycoprotein N-acetylglucosaminyltransferase I; Protein COMPLEX GLYCAN LESS 1
Key Publications

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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