Recombinant Arabidopsis thaliana FACT complex subunit SPT16 (SPT16), partial, Arabidopsis thaliana (Mouse-ear cress), E.coli, N-terminal 6xHis-SUMO-tagged, 36.9kDa, purity >90% as determined by SDS-PAGE.

Recombinant Arabidopsis thaliana FACT complex subunit SPT16 (SPT16), partial

Recombinant Arabidopsis thaliana FACT complex subunit SPT16 (SPT16), partial — Component of the FACT complex, a general chromatin factor that acts to reorganize nucleosomes. Purity >90%.

SKU: BCRECP-004478 Category:
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Product Specifications

Product SkuBCRECP-004478
Product DescriptionRecombinant Arabidopsis thaliana FACT complex subunit SPT16 (SPT16) Protein is expressed from E.coli with N-terminal 6xHis-SUMO-tagged. It contains 890-1074aa. [Accession | O82491].
Uniprot No.O82491
Gene NamesSPT16
PurityGreater than 90% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region890-1074aa
SpeciesArabidopsis thaliana (Mouse-ear cress)
Tag InfoN-terminal 6xHis-SUMO-tagged
Molecular weight36.9kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasDevelopmental Biology

Function

Component of the FACT complex, a general chromatin factor that acts to reorganize nucleosomes. The FACT complex is involved in multiple processes that require DNA as a template such as mRNA elongation, DNA replication and DNA repair. During transcription elongation the FACT complex acts as a histone chaperone that both destabilizes and restores nucleosomal structure.

Biological Context

Subcellular Location: Nucleus; Chromosome
Tissue Specificity: Widely expressed. Present in embryos, shoots and roots, whereas it is not present in terminally differentiated cells such as mature trichoblasts or cells of the root cap (at protein level)

Product Specifications

Recombinant Arabidopsis thaliana FACT complex subunit SPT16 (SPT16), partial is a recombinant protein from Arabidopsis thaliana (Mouse-ear cress), expressed in E.coli, covering amino acids 890-1074aa, with N-terminal 6xHis-SUMO-tagged tag, molecular weight 36.9kDa, purity Greater than 90% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

Recommended applications depend on the target, product format, and product-specific validation data. Refer to the product datasheet or Certificate of Analysis (CoA), or contact BioCrest Sci for application guidance.

Biological Activity: Please contact us to obtain bioactivity data.

Storage: Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shipping & Lead Time: Availability, shipping conditions, and estimated lead time are confirmed in the formal quotation or order confirmation based on the product format, destination, and order requirements.

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Protein Biology

Function

Component of the FACT complex, a general chromatin factor that acts to reorganize nucleosomes. The FACT complex is involved in multiple processes that require DNA as a template such as mRNA elongation, DNA replication and DNA repair. During transcription elongation the FACT complex acts as a histone chaperone that both destabilizes and restores nucleosomal structure. It facilitates the passage of RNA polymerase II and transcription by promoting the dissociation of one histone H2A-H2B dimer from the nucleosome, then subsequently promotes the reestablishment of the nucleosome following the passage of RNA polymerase II (Probable)

Subcellular Location

Nucleus; Chromosome

Tissue Specificity

Widely expressed. Present in embryos, shoots and roots, whereas it is not present in terminally differentiated cells such as mature trichoblasts or cells of the root cap (at protein level)

Subunit

Component of the FACT complex, a stable heterodimer of SPT16 and SSRP

Gene: SPT16  |  Organism: Arabidopsis thaliana  |  Synonyms: Facilitates chromatin transcription complex subunit SPT16

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Quality-control data such as identity, purity, endotoxin level, or biological activity are provided only when specified on this product page, the datasheet, or Certificate of Analysis (CoA). Biological activity is not implied unless explicitly stated.

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