Recombinant Arabidopsis thaliana GRF1-interacting factor 1 (GIF1)

Function

Transcription coactivator that plays a role in the regulation of cell expansion in leaf and cotyledons tissues. Component of a network formed by miR396, the GRFs and their interacting factors (GIFs) acting in the regulation of meristem function, at least partially through the control of cell proliferation (PubMed:19392710). Appears to function synergistically with GRF1 as a transcriptional coactivator. Acts together with GRF5 for the development of appropriate leaf size and shape through the promotion and/or maintenance of cell proliferation activity in leaf primordia. Plays a role in adaxial/abaxial patterning and growth in leaf morphogenesis. GIFs are involved in the positive regulation of cell proliferation of lateral organs in a functionally redundant manner. Together with GATA18/HAN, mediates cotyledon identity by preventing ectopic root formation through the repression of PLT1 expression (PubMed:22669825)

Tissue Specificity

Strongly expressed in actively growing and developing tissues, such as roots, upper stems, and shoot tips and flower buds. Also expressed in mature flowers. Not expressed in the shoot apical meristem (SAM). Highly accumulated in the proximal part of leaf primordia, in the key proliferative zone at the junction region between the leaf blade and leaf petiole

Subunit

Interacts with GRF1, GRF2, GRF5 and GRF9

• Kim J.H. et al. A transcriptional coactivator, AtGIF1, is involved in regulating leaf growth and morphology in Arabidopsis. — Proc. Natl. Acad. Sci. U.S.A. (2004)
• Tabata S. et al. Sequence and analysis of chromosome 5 of the plant Arabidopsis thaliana. — Nature (2000)
• Cheng C.Y. et al. Araport11: a complete reannotation of the Arabidopsis thaliana reference genome. — Plant J. (2017)
• Yamada K. et al. Empirical analysis of transcriptional activity in the Arabidopsis genome. — Science (2003)
• Horiguchi G. et al. The transcription factor AtGRF5 and the transcription coactivator AN3 regulate cell proliferation in leaf primordia of Arabidopsis thaliana. — Plant J. (2005)
• Fujikura U. et al. Coordination of cell proliferation and cell expansion mediated by ribosome-related processes in the leaves of Arabidopsis thaliana. — Plant J. (2009)
• Lee B.H. et al. The Arabidopsis GRF-INTERACTING FACTOR gene family performs an overlapping function in determining organ size as well as multiple developmental properties. — Plant Physiol. (2009)
• Wang L. et al. miR396-targeted AtGRF transcription factors are required for coordination of cell division and differentiation during leaf development in Arabidopsis. — J. Exp. Bot. (2011)