Recombinant Arabidopsis thaliana Root meristem growth factor 1 (RGF1)

Function

Acts as a peptide hormone recognized by receptors (e.g. RGI1 and RGI2) to trigger signaling events including the regulation of RITF1 expression and leading to the production of reactive oxygen species (ROS) in roots to modulate meristem size (PubMed:31801996). Signaling peptide (root growth factor) that maintains the postembryonic root stem cell niche in a PIN2-traffic dependent manner by regulating the expression levels and patterns of the transcription factor PLETHORA (PLT), mainly at the post-transcriptional level (PubMed:20798316, PubMed:23370719). Maintains the postembryonic root stem cell niche by regulating the expression levels and patterns of the transcription factor PLETHORA (e.g. PLT1 and PLT2), mainly at the post-transcriptional level (PubMed:20798316). Influences circumferential cell number in the root meristem in response to Pi-deprivation (PubMed:25856240). Regulates the pattern of root growth and lateral root development by modulating the length and the number of cortical cells in the root apical meristem (RAM), and the anticlinal asymmetric cell divisions in lateral root initiation cells (PubMed:22307643, PubMed:23370719)

Subcellular Location

Secreted

Tissue Specificity

Expressed in root tips, only in the quiescent center and the columella stem cells

Subunit

Interacts with RGI1; this interaction triggers RGI1 phosphorylation and ubiquitination (PubMed:27229312). Binds to LRR receptor-like serine/threonine-protein kinases RGI1, RGI2 and RGI3 to trigger their dimerization with SERK proteins and subsequent signaling (PubMed:27001831, PubMed:27229311)

• Matsuzaki Y. et al. Secreted peptide signals required for maintenance of root stem cell niche in Arabidopsis. — Science (2010)
• Nakamura Y. et al. Structural analysis of Arabidopsis thaliana chromosome 5. VII. Sequence features of the regions of 1,013,767 bp covered by sixteen physically assigned P1 and TAC clones. — DNA Res. (1998)
• Cheng C.Y. et al. Araport11: a complete reannotation of the Arabidopsis thaliana reference genome. — Plant J. (2017)
• Underwood B.A. et al. Simultaneous high-throughput recombinational cloning of open reading frames in closed and open configurations. — Plant Biotechnol. J. (2006)
• Meng L. et al. CLE-like (CLEL) peptides control the pattern of root growth and lateral root development in Arabidopsis. — Proc. Natl. Acad. Sci. U.S.A. (2012)
• Fernandez A. et al. Transcriptional and functional classification of the GOLVEN/ROOT GROWTH FACTOR/CLE-like signaling peptides reveals their role in lateral root and hair formation. — Plant Physiol. (2013)
• Cederholm H.M. et al. Distinct sensitivities to phosphate deprivation suggest that RGF peptides play disparate roles in Arabidopsis thaliana root development. — New Phytol. (2015)
• Ou Y. et al. RGF1 INSENSITIVE 1 to 5, a group of LRR receptor-like kinases, are essential for the perception of root meristem growth factor 1 in Arabidopsis thaliana. — Cell Res. (2016)