Recombinant Avian infectious bronchitis virus Spike glycoprotein (S), partial, Avian infectious bronchitis virus (strain UK/123/82) (IBV), Mammalian cell, C-terminal 6xHis-tagged, 27kDa, purity >90% as determined by SDS-PAGE.
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Recombinant Avian infectious bronchitis virus Spike glycoprotein (S), partial

Recombinant Avian infectious bronchitis virus Spike glycoprotein (S), partial — S2 is a class I viral fusion protein. Purity >90%.

SKU: BCRECP-004325 Categories: , ,
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Product Specifications

Product SkuBCRECP-004325
Product DescriptionRecombinant Avian infectious bronchitis virus Spike glycoprotein (S) Protein is expressed from Mammalian cell with C-terminal 6xHis-tagged. It contains 300-520aa. [Accession | P30206].
Uniprot No.P30206
Gene NamesS
PurityGreater than 90% as determined by SDS-PAGE.
Expression SystemMammalian cell
Expression Region300-520aa
SpeciesAvian infectious bronchitis virus (strain UK/123/82) (IBV)
Tag InfoC-terminal 6xHis-tagged
Molecular weight27kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasMicrobiology

Function

S2 is a class I viral fusion protein. Under the current model, the protein has at least 3 conformational states: pre-fusion native state, pre-hairpin intermediate state, and post-fusion hairpin state. During viral and target cell membrane fusion, the coiled coil regions (heptad repeats) assume a trimer-of-hairpins structure, positioning the fusion peptide in close proximity to the C-terminal region of the ectodomain.

Biological Context

Subcellular Location: Virion membrane (Peripheral membrane protein); Host endoplasmic reticulum-Golgi intermediate compartment membrane (Peripheral membrane protein)

Product Specifications

Recombinant Avian infectious bronchitis virus Spike glycoprotein (S), partial is a recombinant protein from Avian infectious bronchitis virus (strain UK/123/82) (IBV), expressed in Mammalian cell, covering amino acids 300-520aa, with C-terminal 6xHis-tagged tag, molecular weight 27kDa, purity Greater than 90% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

Recommended applications depend on the target, product format, and product-specific validation data. Refer to the product datasheet or Certificate of Analysis (CoA), or contact BioCrest Sci for application guidance.

Biological Activity: Please contact us to obtain bioactivity data.

Storage: Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shipping & Lead Time: Availability, shipping conditions, and estimated lead time are confirmed in the formal quotation or order confirmation based on the product format, destination, and order requirements.

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Protein Biology

Function

S2 is a class I viral fusion protein. Under the current model, the protein has at least 3 conformational states: pre-fusion native state, pre-hairpin intermediate state, and post-fusion hairpin state. During viral and target cell membrane fusion, the coiled coil regions (heptad repeats) assume a trimer-of-hairpins structure, positioning the fusion peptide in close proximity to the C-terminal region of the ectodomain. The formation of this structure appears to drive apposition and subsequent fusion of viral and target cell membranes (By similarity)

Subcellular Location

Virion membrane (Peripheral membrane protein); Host endoplasmic reticulum-Golgi intermediate compartment membrane (Peripheral membrane protein)

Subunit

Homotrimer; each monomer consists of a S1 and a S2 subunit. The resulting peplomers protrude from the virus surface as spikes (By similarity)

Gene: S  |  Organism: Avian infectious bronchitis virus (strain UK/123/82)  |  Synonyms: E2; Peplomer protein

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How do I order or inquire about this product?

Submit the Online Inquiry form with your required quantity and specifications, or email sales@biocrestsci.com. Available sizes, pricing, stock status, documentation, and estimated lead time are confirmed in the formal quotation.

What is the shipping and delivery time?

Availability, dispatch timing, shipping conditions, and delivery estimates depend on the product format, destination, and order requirements. These details are confirmed in the formal quotation or order confirmation.

How should this product be stored?

Follow the storage and handling instructions in the product-specific datasheet and Certificate of Analysis (CoA). Requirements may vary by product form and formulation; contact BioCrest Sci if product-specific guidance is needed.

What quality controls are performed on your products?

Quality-control data such as identity, purity, endotoxin level, or biological activity are provided only when specified on this product page, the datasheet, or Certificate of Analysis (CoA). Biological activity is not implied unless explicitly stated.

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