Recombinant Bacillus subtilis Biofilm-surface layer protein A (bslA) Protein, Bacillus subtilis (strain 168), in vitro E.coli expression system, N-terminal 10xHis-tagged, 20.8kDa, purity >85% as determined by SDS-PAGE.

Recombinant Bacillus subtilis Biofilm-surface layer protein A (bslA) Protein

Recombinant Bacillus subtilis Biofilm-surface layer protein A (bslA) Protein — Involved in biofilm formation. Purity >85%.

SKU: BCRECP-00418TM Category:
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Product Specifications

Uniprot No.P71014
Gene NamesP71014
PurityGreater than 85% as determined by SDS-PAGE.
Expression Systemin vitro E.coli expression system
Expression Region1-181aa
SpeciesBacillus subtilis (strain 168)
Tag InfoN-terminal 10xHis-tagged
Molecular weight20.8kDa
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Function

Involved in biofilm formation. Self-polymerizes and forms a layer on the surface of biofilms that confers hydrophobicity to the biofilm. The layer is stable and capable of resistance to high mechanical force compression.

Biological Context

Subcellular Location: Secreted; Secreted, cell wall

Product Specifications

Recombinant Bacillus subtilis Biofilm-surface layer protein A (bslA) Protein is a recombinant protein from Bacillus subtilis (strain 168), expressed in in vitro E.coli expression system, covering amino acids 1-181aa, with N-terminal 10xHis-tagged tag, molecular weight 20.8kDa, purity Greater than 85% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

Recommended applications depend on the target, product format, and product-specific validation data. Refer to the product datasheet or Certificate of Analysis (CoA), or contact BioCrest Sci for application guidance.

Biological Activity: Not implied unless activity data are explicitly listed on this product page, the datasheet, or the CoA.

Storage: Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shipping & Lead Time: Availability, shipping conditions, and estimated lead time are confirmed in the formal quotation or order confirmation based on the product format, destination, and order requirements.

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Protein Biology

Function

Involved in biofilm formation (PubMed:18978066, PubMed:21742882). Self-polymerizes and forms a layer on the surface of biofilms that confers hydrophobicity to the biofilm (PubMed:22571672, PubMed:23904481). The layer is stable and capable of resistance to high mechanical force compression (PubMed:28701036). Required for complex colony architecture (PubMed:18978066). May function synergistically with exopolysaccharides and TasA amyloid fibers to facilitate the assembly of the biofilm matrix (PubMed:21742882)

Subcellular Location

Secreted; Secreted, cell wall

Subunit

Forms polymers

Gene: bslA  |  Organism: Bacillus subtilis (strain 168)  |  Synonyms: ORF-1
Key Publications

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Frequently Asked Questions

How do I order or inquire about this product?

Submit the Online Inquiry form with your required quantity and specifications, or email sales@biocrestsci.com. Available sizes, pricing, stock status, documentation, and estimated lead time are confirmed in the formal quotation.

What is the shipping and delivery time?

Availability, dispatch timing, shipping conditions, and delivery estimates depend on the product format, destination, and order requirements. These details are confirmed in the formal quotation or order confirmation.

How should this product be stored?

Follow the storage and handling instructions in the product-specific datasheet and Certificate of Analysis (CoA). Requirements may vary by product form and formulation; contact BioCrest Sci if product-specific guidance is needed.

What quality controls are performed on your products?

Quality-control data such as identity, purity, endotoxin level, or biological activity are provided only when specified on this product page, the datasheet, or Certificate of Analysis (CoA). Biological activity is not implied unless explicitly stated.

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