Recombinant Bacillus subtilis Penicillin-binding protein 3 (pbpC), partial, Bacillus subtilis (strain 168), E.coli, N-terminal 6xHis-tagged, 29.2kDa, purity >90% as determined by SDS-PAGE.

Recombinant Bacillus subtilis Penicillin-binding protein 3 (pbpC), partial

Recombinant Bacillus subtilis Penicillin-binding protein 3 (pbpC), partial — Penicillin-binding proteins (PBPs) function in the late steps of murein biosynthesis (Probable). Purity >90%.

SKU: BCRECP-004029 Category:
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Product Specifications

Product SkuBCRECP-004029
Product DescriptionRecombinant Bacillus subtilis Penicillin-binding protein 3 (pbpC) Protein is expressed from E.coli with N-terminal 6xHis-tagged. It contains 21-240aa. [Accession | P42971].
Uniprot No.P42971
Gene NamespbpC
PurityGreater than 90% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region21-240aa
SpeciesBacillus subtilis (strain 168)
Tag InfoN-terminal 6xHis-tagged
Molecular weight29.2kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasMicrobiology

Function

Penicillin-binding proteins (PBPs) function in the late steps of murein biosynthesis (Probable). Probably required for both cortical and vegetative peptidoglycan synthesis (Probable). Although not usually required for cell division, in the absence of PBP 2B (pbpB) it becomes essential.

Biological Context

Subcellular Location: Cell membrane (Single-pass membrane protein); Forespore inner membrane (Single-pass membrane protein); Forespore outer membrane (Single-pass membrane protein); Membrane raft (Single-pass membrane protein)
Pathway: Cell wall biogenesis; peptidoglycan biosynthesis

Product Specifications

Recombinant Bacillus subtilis Penicillin-binding protein 3 (pbpC), partial is a recombinant protein from Bacillus subtilis (strain 168), expressed in E.coli, covering amino acids 21-240aa, with N-terminal 6xHis-tagged tag, molecular weight 29.2kDa, purity Greater than 90% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

Related products

Protein Biology

Function

Penicillin-binding proteins (PBPs) function in the late steps of murein biosynthesis (Probable). Probably required for both cortical and vegetative peptidoglycan synthesis (Probable). Although not usually required for cell division, in the absence of PBP 2B (pbpB) it becomes essential. Confers resistance to oxacillin and cephalexin (PubMed:28792086)

Subcellular Location

Cell membrane (Single-pass membrane protein); Forespore inner membrane (Single-pass membrane protein); Forespore outer membrane (Single-pass membrane protein); Membrane raft (Single-pass membrane protein)

Pathway

Cell wall biogenesis; peptidoglycan biosynthesis

Gene: pbpC  |  Organism: Bacillus subtilis (strain 168)  |  Synonyms: PSPB20; Penicillin-binding protein C
Key Publications

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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