Recombinant Bacillus subtilis Subtilisin E (aprE)

Recombinant Bacillus subtilis Subtilisin E (aprE) — recombinant protein from Bacillus subtilis (strain 168), expressed in E.coli. Purity >90%.

SKU: BCRECP-002121 Category:

Product Specifications

Product SkuBCRECP-002121
Product DescriptionRecombinant Bacillus subtilis Subtilisin E (aprE) Protein is expressed from E.coli with N-terminal 6xHis-SUMO-tagged. It contains 107-381aa. [Accession | P04189].
Uniprot No.P04189
Gene NamesaprE
PurityGreater than 90% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region107-381aa
SpeciesBacillus subtilis (strain 168)
Tag InfoN-terminal 6xHis-SUMO-tagged
Molecular weight43.7kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasOthers

Function

An extracellular alkaline serine protease, it catalyzes the hydrolysis of proteins and peptide amides.

Biological Context

Subcellular Location: Secreted

Product Specifications

Recombinant Bacillus subtilis Subtilisin E (aprE) is a recombinant protein from Bacillus subtilis (strain 168), expressed in E.coli, covering amino acids 107-381aa, with N-terminal 6xHis-SUMO-tagged tag, molecular weight 43.7kDa, purity Greater than 90% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

Recommended applications depend on the target, product format, and product-specific validation data. Refer to the product datasheet or Certificate of Analysis (CoA), or contact BioCrest Sci for application guidance.

Biological Activity: Please contact us to obtain bioactivity data.

Storage: Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shipping & Lead Time: Availability, shipping conditions, and estimated lead time are confirmed in the formal quotation or order confirmation based on the product format, destination, and order requirements.

Frequently Asked Questions

How do I order or inquire about this product?

Submit the Online Inquiry form with your required quantity and specifications, or email sales@biocrestsci.com. Available sizes, pricing, stock status, documentation, and estimated lead time are confirmed in the formal quotation.

What is the shipping and delivery time?

Availability, dispatch timing, shipping conditions, and delivery estimates depend on the product format, destination, and order requirements. These details are confirmed in the formal quotation or order confirmation.

How should this product be stored?

Follow the storage and handling instructions in the product-specific datasheet and Certificate of Analysis (CoA). Requirements may vary by product form and formulation; contact BioCrest Sci if product-specific guidance is needed.

What quality controls are performed on your products?

Quality-control data such as identity, purity, endotoxin level, or biological activity are provided only when specified on this product page, the datasheet, or Certificate of Analysis (CoA). Biological activity is not implied unless explicitly stated.

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