Recombinant Bacillus thuringiensis subsp. kurstaki Pesticidal crystal protein Cry2Aa (cry2Aa), partial, Bacillus thuringiensis subsp. Kurstaki, E.coli, N-terminal 10xHis-tagged and C-terminal Myc-tagged, 29.7kDa, purity >90% as determined by SDS-PAGE.

Recombinant Bacillus thuringiensis subsp. kurstaki Pesticidal crystal protein Cry2Aa (cry2Aa), partial

Recombinant Bacillus thuringiensis subsp. kurstaki Pesticidal crystal protein Cry2Aa (cry2Aa), partial — recombinant protein from Bacillus thuringiensis subsp. Kurstaki, expressed in E.coli. Purity >90%.

SKU: BCRECP-002443 Category:
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Product Specifications

Product SkuBCRECP-002443
Product DescriptionRecombinant Bacillus thuringiensis subsp. kurstaki Pesticidal crystal protein Cry2Aa (cry2Aa) Protein is expressed from E.coli with N-terminal 10xHis-tagged and C-terminal Myc-tagged. It contains 267-472aa. [Accession | P0A377].
Uniprot No.P0A377
Gene Namescry2Aa
PurityGreater than 90% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region267-472aa
SpeciesBacillus thuringiensis subsp. Kurstaki
Tag InfoN-terminal 10xHis-tagged and C-terminal Myc-tagged
Molecular weight29.7kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasOthers

Function

Promotes colloidosmotic lysis by binding to the midgut epithelial cells of both dipteran (Aedes aegypti) and lepidopteran (Manduca sexta) larvae.

Product Specifications

Recombinant Bacillus thuringiensis subsp. kurstaki Pesticidal crystal protein Cry2Aa (cry2Aa), partial is a recombinant protein from Bacillus thuringiensis subsp. Kurstaki, expressed in E.coli, covering amino acids 267-472aa, with N-terminal 10xHis-tagged and C-terminal Myc-tagged tag, molecular weight 29.7kDa, purity Greater than 90% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

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Protein Biology

Function

Promotes colloidosmotic lysis by binding to the midgut epithelial cells of both dipteran (Aedes aegypti) and lepidopteran (Manduca sexta) larvae

Gene: cry2Aa  |  Organism: Bacillus thuringiensis subsp. kurstaki  |  Synonyms: 71 kDa crystal protein; Crystaline entomocidal protoxin; Insecticidal delta-endotoxin CryIIA(a); Mosquito factor; P2 crystal protein

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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