Recombinant Bordetella pertussis BrkA autotransporter (brkA), Bordetella pertussis (strain Tohama I / ATCC BAA-589 / NCTC 13251), E.coli, N-terminal 10xHis-tagged and C-terminal Myc-tagged, 110.8kDa, purity >85% as determined by SDS-PAGE.

Recombinant Bordetella pertussis BrkA autotransporter (brkA)

Recombinant Bordetella pertussis BrkA autotransporter (brkA) — recombinant protein from Bordetella pertussis (strain Tohama I / ATCC BAA-589 / NCTC 13251), expressed in E.coli. Purity >85%.

SKU: BCRECP-002233 Category:
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Product Specifications

Product SkuBCRECP-002233
Product DescriptionRecombinant Bordetella pertussis BrkA autotransporter (brkA) Protein is expressed from E.coli with N-terminal 10xHis-tagged and C-terminal Myc-tagged. It contains 1-1010aa. [Accession | Q45340].
Uniprot No.Q45340
Gene NamesbrkA
PurityGreater than 85% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region1-1010aa
SpeciesBordetella pertussis (strain Tohama I / ATCC BAA-589 / NCTC 13251)
Tag InfoN-terminal 10xHis-tagged and C-terminal Myc-tagged
Molecular weight110.8kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasOthers

Function

Inhibits the classical pathway of complement activation and prevents accumulation of deposited C4.

Biological Context

Subcellular Location: Cell outer membrane (Multi-pass membrane protein)

Product Specifications

Recombinant Bordetella pertussis BrkA autotransporter (brkA) is a recombinant protein from Bordetella pertussis (strain Tohama I / ATCC BAA-589 / NCTC 13251), expressed in E.coli, covering amino acids 1-1010aa, with N-terminal 10xHis-tagged and C-terminal Myc-tagged tag, molecular weight 110.8kDa, purity Greater than 85% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

Recommended applications depend on the target, product format, and product-specific validation data. Refer to the product datasheet or Certificate of Analysis (CoA), or contact BioCrest Sci for application guidance.

Biological Activity: Please contact us to obtain bioactivity data.

Storage: Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shipping & Lead Time: Availability, shipping conditions, and estimated lead time are confirmed in the formal quotation or order confirmation based on the product format, destination, and order requirements.

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Protein Biology

Function

Inhibits the classical pathway of complement activation and prevents accumulation of deposited C4

Subcellular Location

Cell outer membrane (Multi-pass membrane protein)

Gene: brkA  |  Organism: Bordetella pertussis (strain Tohama I / ATCC BAA-589 / NCTC 13251)
Key Publications

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Frequently Asked Questions

How do I order or inquire about this product?

Submit the Online Inquiry form with your required quantity and specifications, or email sales@biocrestsci.com. Available sizes, pricing, stock status, documentation, and estimated lead time are confirmed in the formal quotation.

What is the shipping and delivery time?

Availability, dispatch timing, shipping conditions, and delivery estimates depend on the product format, destination, and order requirements. These details are confirmed in the formal quotation or order confirmation.

How should this product be stored?

Follow the storage and handling instructions in the product-specific datasheet and Certificate of Analysis (CoA). Requirements may vary by product form and formulation; contact BioCrest Sci if product-specific guidance is needed.

What quality controls are performed on your products?

Quality-control data such as identity, purity, endotoxin level, or biological activity are provided only when specified on this product page, the datasheet, or Certificate of Analysis (CoA). Biological activity is not implied unless explicitly stated.

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