Recombinant Bordetella pertussis Pertussis toxin subunit 1 (ptxA), Bordetella pertussis (strain Tohama I / ATCC BAA-589 / NCTC 13251), E.coli, N-terminal 6xHis-B2M-tagged, 40.8kDa, purity >85% as determined by SDS-PAGE.

Recombinant Bordetella pertussis Pertussis toxin subunit 1 (ptxA)

Recombinant Bordetella pertussis Pertussis toxin subunit 1 (ptxA) — S1 is an NAD-dependent ADP-ribosyltransferase, which plays a crucial role in the pathogenesis of B. Purity >85%.

SKU: BCRECP-004213 Category:
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Product Specifications

Product SkuBCRECP-004213
Product DescriptionRecombinant Bordetella pertussis Pertussis toxin subunit 1 (ptxA) Protein is expressed from E.coli with N-terminal 6xHis-B2M-tagged. It contains 35-269aa. [Accession | P04977].
Uniprot No.P04977
Gene NamesptxA
PurityGreater than 85% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region35-269aa
SpeciesBordetella pertussis (strain Tohama I / ATCC BAA-589 / NCTC 13251)
Tag InfoN-terminal 6xHis-B2M-tagged
Molecular weight40.8kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasOthers

Function

S1 is an NAD-dependent ADP-ribosyltransferase, which plays a crucial role in the pathogenesis of B.pertussis causing disruption of normal host cellular regulation. It catalyzes the ADP-ribosylation of a cysteine in the alpha subunit of host heterotrimeric G proteins. In the absence of G proteins it also catalyzes the cleavage of NAD(+) into ADP-ribose and nicotinamide.

Biological Context

Subcellular Location: Secreted

Product Specifications

Recombinant Bordetella pertussis Pertussis toxin subunit 1 (ptxA) is a recombinant protein from Bordetella pertussis (strain Tohama I / ATCC BAA-589 / NCTC 13251), expressed in E.coli, covering amino acids 35-269aa, with N-terminal 6xHis-B2M-tagged tag, molecular weight 40.8kDa, purity Greater than 85% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

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Protein Biology

Function

S1 is an NAD-dependent ADP-ribosyltransferase, which plays a crucial role in the pathogenesis of B.pertussis causing disruption of normal host cellular regulation. It catalyzes the ADP-ribosylation of a cysteine in the alpha subunit of host heterotrimeric G proteins. In the absence of G proteins it also catalyzes the cleavage of NAD(+) into ADP-ribose and nicotinamide. It irreversibly uncouples the G-alpha GTP-binding proteins from their membrane receptors

Subcellular Location

Secreted

Subunit

Pertussis toxin contains five different chains, S1-S5. They are organized into 2 functional subunits: A, composed of S1 (which is toxic) and B, containing S2, S3, S5, and two copies of S4 (B binds to the membrane receptors). Dimers of S2-S4 and S3-S4 are held together by S5

Gene: ptxA  |  Organism: Bordetella pertussis (strain Tohama I / ATCC BAA-589 / NCTC 13251)  |  Synonyms: Islet-activating protein S1; NAD-dependent ADP-ribosyltransferase
Key Publications

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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