Recombinant Bothrops atrox Zinc metalloproteinase atroxlysin-1, Bothrops atrox (Barba amarilla) (Fer-de-lance), E.coli, N-terminal 10xHis-tagged and C-terminal Myc-tagged, 30.4kDa, purity >85% as determined by SDS-PAGE.

Recombinant Bothrops atrox Zinc metalloproteinase atroxlysin-1

Recombinant Bothrops atrox Zinc metalloproteinase atroxlysin-1 — Snake venom zinc metalloproteinase, which causes strong hemorrhagy and dermonecrosis by degrading the extracellular matrix. Purity >85%.

SKU: BCRECP-003554 Category:
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Product Specifications

Product SkuBCRECP-003554
Product DescriptionRecombinant Bothrops atrox Zinc metalloproteinase atroxlysin-1 Protein is expressed from E.coli with N-terminal 10xHis-tagged and C-terminal Myc-tagged. It contains 1-202aa. [Accession | P85420].
Uniprot No.P85420
PurityGreater than 85% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region1-202aa
SpeciesBothrops atrox (Barba amarilla) (Fer-de-lance)
Tag InfoN-terminal 10xHis-tagged and C-terminal Myc-tagged
Molecular weight30.4kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasOthers

Function

Snake venom zinc metalloproteinase, which causes strong hemorrhagy and dermonecrosis by degrading the extracellular matrix. Its strong hemorrhagic activity is independent of binding to extracellular matrix (ECM) proteins and is instead due to its high catalytic activity, in contrast to P-III metalloproteinases, which bind to the ECM but have lower catalytic activity. Its unique dermonecrotic action may be explained by its efficacy in laminin hydrolysis (Probable).

Biological Context

Subcellular Location: Secreted
Tissue Specificity: Expressed by the venom gland

Product Specifications

Recombinant Bothrops atrox Zinc metalloproteinase atroxlysin-1 is a recombinant protein from Bothrops atrox (Barba amarilla) (Fer-de-lance), expressed in E.coli, covering amino acids 1-202aa, with N-terminal 10xHis-tagged and C-terminal Myc-tagged tag, molecular weight 30.4kDa, purity Greater than 85% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

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Protein Biology

Function

Snake venom zinc metalloproteinase, which causes strong hemorrhagy and dermonecrosis by degrading the extracellular matrix (PubMed:20102699, PubMed:28767072). Its strong hemorrhagic activity is independent of binding to extracellular matrix (ECM) proteins and is instead due to its high catalytic activity, in contrast to P-III metalloproteinases, which bind to the ECM but have lower catalytic activity (PubMed:28767072). Its unique dermonecrotic action may be explained by its efficacy in laminin hydrolysis (Probable). Cleaves Xaa-Leu bonds (PubMed:20102699). Hydrolyzes fibrinogen, fibrin, fibronectin (FN1), type I collagen, type IV collagen, laminin, nidogen, integrin alpha-7/beta-1 (ITGA7/ITGB1) and integrin alpha-1/beta-1 (ITGA1/ITGB1) (PubMed:20102699, PubMed:28767072). Binds to fibronectin (FN1) and fibrinogen (PubMed:20102699). Shows no or very weak binding to type I collagen and laminin, and no binding to type IV collagen and nidogen (PubMed:20102699, PubMed:28767072). Inhibits ADP- and collagen-induced platelet aggregation both in the presence (IC(50)=1.4 uM for collagen) and in the absence (IC(50)=2.2 uM for collagen) of cofactors (PubMed:20102699)

Subcellular Location

Secreted

Tissue Specificity

Expressed by the venom gland

Subunit

Monomer

Organism: Bothrops atrox  |  Synonyms: Atroxlysin-Ia; BATXSVMPI5

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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