Recombinant Bovine Adrenodoxin, mitochondrial (FDX1)

Recombinant Bovine Adrenodoxin, mitochondrial (FDX1) — Essential for the synthesis of various steroid hormones. Purity >85%.

SKU: BCRECP-002718 Category:

Product Specifications

Product SkuBCRECP-002718
Product DescriptionRecombinant Bovine Adrenodoxin, mitochondrial (FDX1) Protein is expressed from E.coli with N-terminal 10xHis-tagged. It contains 59-186aa. [Accession | P00257].
Uniprot No.P00257
Gene NamesFDX1
PurityGreater than 85% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region59-186aa
SpeciesBos taurus (Bovine)
Tag InfoN-terminal 10xHis-tagged
Molecular weight19.5kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasMetabolism

Function

Essential for the synthesis of various steroid hormones. Participates in the reduction of mitochondrial cytochrome P450 for steroidogenesis. Transfers electrons from adrenodoxin reductase to CYP11A1, a cytochrome P450 that catalyzes cholesterol side-chain cleavage to produce pregnenolone, the precursor of most steroid hormones.

Biological Context

Subcellular Location: Mitochondrion matrix
Tissue Specificity: Detected in adrenal cortex and corpus luteum (at protein level)

Product Specifications

Recombinant Bovine Adrenodoxin, mitochondrial (FDX1) is a recombinant protein from Bos taurus (Bovine), expressed in E.coli, covering amino acids 59-186aa, with N-terminal 10xHis-tagged tag, molecular weight 19.5kDa, purity Greater than 85% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

Recommended applications depend on the target, product format, and product-specific validation data. Refer to the product datasheet or Certificate of Analysis (CoA), or contact BioCrest Sci for application guidance.

Biological Activity: Please contact us to obtain bioactivity data.

Storage: Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shipping & Lead Time: Availability, shipping conditions, and estimated lead time are confirmed in the formal quotation or order confirmation based on the product format, destination, and order requirements.

Protein Biology

Function

Essential for the synthesis of various steroid hormones. Participates in the reduction of mitochondrial cytochrome P450 for steroidogenesis. Transfers electrons from adrenodoxin reductase to CYP11A1, a cytochrome P450 that catalyzes cholesterol side-chain cleavage to produce pregnenolone, the precursor of most steroid hormones. Does not form a ternary complex with adrenodoxin reductase and CYP11A1 but shuttles between the two enzymes to transfer electrons

Subcellular Location

Mitochondrion matrix

Tissue Specificity

Detected in adrenal cortex and corpus luteum (at protein level)

Subunit

Interacts with CYP11A1

Gene: FDX1  |  Organism: Bos taurus  |  Synonyms: Adrenal ferredoxin; Ferredoxin-1; Hepato-ferredoxin
Key Publications

Frequently Asked Questions

How do I order or inquire about this product?

Submit the Online Inquiry form with your required quantity and specifications, or email sales@biocrestsci.com. Available sizes, pricing, stock status, documentation, and estimated lead time are confirmed in the formal quotation.

What is the shipping and delivery time?

Availability, dispatch timing, shipping conditions, and delivery estimates depend on the product format, destination, and order requirements. These details are confirmed in the formal quotation or order confirmation.

How should this product be stored?

Follow the storage and handling instructions in the product-specific datasheet and Certificate of Analysis (CoA). Requirements may vary by product form and formulation; contact BioCrest Sci if product-specific guidance is needed.

What quality controls are performed on your products?

Quality-control data such as identity, purity, endotoxin level, or biological activity are provided only when specified on this product page, the datasheet, or Certificate of Analysis (CoA). Biological activity is not implied unless explicitly stated.

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