Recombinant Bovine Cation-independent mannose-6-phosphate receptor (IGF2R), partial, Bos taurus (Bovine), E.coli, N-terminal 6xHis-tagged, 22.4kDa, purity >90% as determined by SDS-PAGE.
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Recombinant Bovine Cation-independent mannose-6-phosphate receptor (IGF2R), partial

Recombinant Bovine Cation-independent mannose-6-phosphate receptor (IGF2R), partial — Mediates the transport of phosphorylated lysosomal enzymes from the Golgi complex and the cell surface to lysosomes. Purity >90%.

SKU: BCRECP-003091 Categories: ,
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Product Specifications

Product SkuBCRECP-003091
Product DescriptionRecombinant Bovine Cation-independent mannose-6-phosphate receptor (IGF2R) Protein is expressed from E.coli with N-terminal 6xHis-tagged. It contains 628-772aa. [Accession | P08169].
Uniprot No.P08169
Gene NamesIGF2R
PurityGreater than 90% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region628-772aa
SpeciesBos taurus (Bovine)
Tag InfoN-terminal 6xHis-tagged
Molecular weight22.4kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasImmunology

Function

Mediates the transport of phosphorylated lysosomal enzymes from the Golgi complex and the cell surface to lysosomes. Lysosomal enzymes bearing phosphomannosyl residues bind specifically to mannose-6-phosphate receptors in the Golgi apparatus and the resulting receptor-ligand complex is transported to an acidic prelysosomal compartment where the low pH mediates the dissociation of the complex. The receptor is then recycled back to the Golgi for another round of trafficking through its binding to the retromer.

Biological Context

Subcellular Location: Golgi apparatus membrane (Single-pass type I membrane protein); Endosome membrane (Single-pass type I membrane protein)

Product Specifications

Recombinant Bovine Cation-independent mannose-6-phosphate receptor (IGF2R), partial is a recombinant protein from Bos taurus (Bovine), expressed in E.coli, covering amino acids 628-772aa, with N-terminal 6xHis-tagged tag, molecular weight 22.4kDa, purity Greater than 90% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

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Protein Biology

Function

Mediates the transport of phosphorylated lysosomal enzymes from the Golgi complex and the cell surface to lysosomes. Lysosomal enzymes bearing phosphomannosyl residues bind specifically to mannose-6-phosphate receptors in the Golgi apparatus and the resulting receptor-ligand complex is transported to an acidic prelysosomal compartment where the low pH mediates the dissociation of the complex. The receptor is then recycled back to the Golgi for another round of trafficking through its binding to the retromer. This receptor also binds IGF2. Acts as a positive regulator of T-cell coactivation by binding DPP4

Subcellular Location

Golgi apparatus membrane (Single-pass type I membrane protein); Endosome membrane (Single-pass type I membrane protein)

Subunit

Binds HA-I and HA-II plasma membrane adapters (PubMed:2545438). Interacts with DPP4; the interaction is direct. Binds GGA1, GGA2 and GGA3 (By similarity). Interacts with the heterotrimeric retromer cargo-selective complex (CSC), formed by VPS26 (VPS26A or VPS26B), VPS29 and VPS35; which is involved in retrograde trafficking of the receptor from endosomes to the Golgi apparatus (By similarity). Interacts with SNX32; the interaction is involved in intracellular trafficking of the receptor (By similarity)

Gene: IGF2R  |  Organism: Bos taurus  |  Synonyms: 300 kDa mannose 6-phosphate receptor; Insulin-like growth factor 2 receptor; Insulin-like growth factor II receptor
Key Publications

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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