Recombinant Bovine Cell death activator CIDE-B (CIDEB), Bos taurus (Bovine), E.coli, N-terminal MBP-tagged and C-terminal 6xHis-tagged, 68.4kDa, purity >90% as determined by SDS-PAGE.

Recombinant Bovine Cell death activator CIDE-B (CIDEB)

Recombinant Bovine Cell death activator CIDE-B (CIDEB) — Lipid transferase specifically expressed in hepatocytes, which promotes unilocular lipid droplet formation by mediating lipid droplet fusion. Purity >90%.

SKU: BCRECP-002378 Category:
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Product Specifications

Product SkuBCRECP-002378
Product DescriptionRecombinant Bovine Cell death activator CIDE-B (CIDEB) Protein is expressed from E.coli with N-terminal MBP-tagged and C-terminal 6xHis-tagged. It contains 1-219aa. [Accession | Q3T191].
Uniprot No.Q3T191
Gene NamesCIDEB
PurityGreater than 90% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region1-219aa
SpeciesBos taurus (Bovine)
Tag InfoN-terminal MBP-tagged and C-terminal 6xHis-tagged
Molecular weight68.4kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasCancer

Function

Lipid transferase specifically expressed in hepatocytes, which promotes unilocular lipid droplet formation by mediating lipid droplet fusion. Lipid droplet fusion promotes their enlargement, restricting lipolysis and favoring lipid storage. Localizes on the lipid droplet surface, at focal contact sites between lipid droplets, and mediates atypical lipid droplet fusion by promoting directional net neutral lipid transfer from the smaller to larger lipid droplets.

Biological Context

Subcellular Location: Lipid droplet; Endoplasmic reticulum membrane (Peripheral membrane protein); Golgi apparatus; Cytoplasmic vesicle, COPI-coated vesicle

Product Specifications

Recombinant Bovine Cell death activator CIDE-B (CIDEB) is a recombinant protein from Bos taurus (Bovine), expressed in E.coli, covering amino acids 1-219aa, with N-terminal MBP-tagged and C-terminal 6xHis-tagged tag, molecular weight 68.4kDa, purity Greater than 90% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

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Protein Biology

Function

Lipid transferase specifically expressed in hepatocytes, which promotes unilocular lipid droplet formation by mediating lipid droplet fusion. Lipid droplet fusion promotes their enlargement, restricting lipolysis and favoring lipid storage (By similarity). Localizes on the lipid droplet surface, at focal contact sites between lipid droplets, and mediates atypical lipid droplet fusion by promoting directional net neutral lipid transfer from the smaller to larger lipid droplets. The transfer direction may be driven by the internal pressure difference between the contacting lipid droplet pair (By similarity). Promotes lipid exchange and lipid droplet fusion in both small and large lipid droplet-containing hepatocytes. In addition to its role in lipid droplet fusion, also involved in cytoplasmic vesicle biogenesis and transport. Required for very-low-density lipoprotein (VLDL) lipidation and maturation. Probably involved in the biogenesis of VLDL transport vesicles by forming a COPII vesicle coat and facilitating the formation of endoplasmic reticulum-derived large vesicles. Also involved in sterol-regulated export of the SCAP-SREBP complex, composed of SCAP, SREBF1/SREBP1 and SREBF2/SREBP2, by promoting loading of SCAP-SREBP into COPII vesicles. May also activate apoptosis (By similarity)

Subcellular Location

Lipid droplet; Endoplasmic reticulum membrane (Peripheral membrane protein); Golgi apparatus; Cytoplasmic vesicle, COPI-coated vesicle

Subunit

Interacts with DFFA. Interacts with DFFB; inhibited by DFFB (By similarity). Interacts with APOB. Interacts with PREB/SEC12; facilitating loading of SCAP-SREBP into COPII vesicles (By similarity)

Gene: CIDEB  |  Organism: Bos taurus  |  Synonyms: Cell death-inducing DFFA-like effector B

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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