Recombinant Bovine Palmitoyl-protein thioesterase 1 (PPT1), Bos taurus (Bovine), E.coli, N-terminal 10xHis-tagged and C-terminal Myc-tagged, 38.9kDa, purity >85% as determined by SDS-PAGE.
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Recombinant Bovine Palmitoyl-protein thioesterase 1 (PPT1)

Recombinant Bovine Palmitoyl-protein thioesterase 1 (PPT1) — Has thioesterase activity against fatty acid thioesters with 14 -18 carbons, including palmitoyl-CoA, S-palmitoyl-N-acetylcysteamine, and palmitoylated proteins. Purity >85%.

SKU: BCRECP-004146 Categories: ,
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Product Specifications

Product SkuBCRECP-004146
Product DescriptionRecombinant Bovine Palmitoyl-protein thioesterase 1 (PPT1) Protein is expressed from E.coli with N-terminal 10xHis-tagged and C-terminal Myc-tagged. It contains 28-306aa. [Accession | P45478].
Uniprot No.P45478
Gene NamesPPT1
PurityGreater than 85% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region28-306aa
SpeciesBos taurus (Bovine)
Tag InfoN-terminal 10xHis-tagged and C-terminal Myc-tagged
Molecular weight38.9kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasNeuroscience

Function

Has thioesterase activity against fatty acid thioesters with 14 -18 carbons, including palmitoyl-CoA, S-palmitoyl-N-acetylcysteamine, and palmitoylated proteins. In contrast to PPT2, PPT1 can hydrolyze palmitoylated proteins and palmitoylcysteine.

Biological Context

Subcellular Location: Lysosome; Secreted; Golgi apparatus; Endoplasmic reticulum
Tissue Specificity: Spleen, brain, seminal vesicle, and testis. Lower levels of activity in liver, heart, lung, and skeletal muscle

Product Specifications

Recombinant Bovine Palmitoyl-protein thioesterase 1 (PPT1) is a recombinant protein from Bos taurus (Bovine), expressed in E.coli, covering amino acids 28-306aa, with N-terminal 10xHis-tagged and C-terminal Myc-tagged tag, molecular weight 38.9kDa, purity Greater than 85% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

Recommended applications depend on the target, product format, and product-specific validation data. Refer to the product datasheet or Certificate of Analysis (CoA), or contact BioCrest Sci for application guidance.

Biological Activity: Please contact us to obtain bioactivity data.

Storage: Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shipping & Lead Time: Availability, shipping conditions, and estimated lead time are confirmed in the formal quotation or order confirmation based on the product format, destination, and order requirements.

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Protein Biology

Function

Has thioesterase activity against fatty acid thioesters with 14 -18 carbons, including palmitoyl-CoA, S-palmitoyl-N-acetylcysteamine, and palmitoylated proteins (PubMed:7916016). In contrast to PPT2, PPT1 can hydrolyze palmitoylated proteins and palmitoylcysteine (By similarity)

Subcellular Location

Lysosome; Secreted; Golgi apparatus; Endoplasmic reticulum

Tissue Specificity

Spleen, brain, seminal vesicle, and testis. Lower levels of activity in liver, heart, lung, and skeletal muscle

Subunit

Interacts with CLN5, ATP5F1A and ATP5F1B

Gene: PPT1  |  Organism: Bos taurus  |  Synonyms: Palmitoyl-protein hydrolase 1

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How do I order or inquire about this product?

Submit the Online Inquiry form with your required quantity and specifications, or email sales@biocrestsci.com. Available sizes, pricing, stock status, documentation, and estimated lead time are confirmed in the formal quotation.

What is the shipping and delivery time?

Availability, dispatch timing, shipping conditions, and delivery estimates depend on the product format, destination, and order requirements. These details are confirmed in the formal quotation or order confirmation.

How should this product be stored?

Follow the storage and handling instructions in the product-specific datasheet and Certificate of Analysis (CoA). Requirements may vary by product form and formulation; contact BioCrest Sci if product-specific guidance is needed.

What quality controls are performed on your products?

Quality-control data such as identity, purity, endotoxin level, or biological activity are provided only when specified on this product page, the datasheet, or Certificate of Analysis (CoA). Biological activity is not implied unless explicitly stated.

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