Recombinant Bovine Palmitoyl-protein thioesterase 1 (PPT1), Bos taurus (Bovine), E.coli, N-terminal 10xHis-tagged and C-terminal Myc-tagged, 38.9kDa, purity >85% as determined by SDS-PAGE.
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Recombinant Bovine Palmitoyl-protein thioesterase 1 (PPT1)

Recombinant Bovine Palmitoyl-protein thioesterase 1 (PPT1) — Has thioesterase activity against fatty acid thioesters with 14 -18 carbons, including palmitoyl-CoA, S-palmitoyl-N-acetylcysteamine, and palmitoylated proteins. Purity >85%.

SKU: BCRECP-004146 Categories: ,
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Product Specifications

Product SkuBCRECP-004146
Product DescriptionRecombinant Bovine Palmitoyl-protein thioesterase 1 (PPT1) Protein is expressed from E.coli with N-terminal 10xHis-tagged and C-terminal Myc-tagged. It contains 28-306aa. [Accession | P45478].
Uniprot No.P45478
Gene NamesPPT1
PurityGreater than 85% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region28-306aa
SpeciesBos taurus (Bovine)
Tag InfoN-terminal 10xHis-tagged and C-terminal Myc-tagged
Molecular weight38.9kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasNeuroscience

Function

Has thioesterase activity against fatty acid thioesters with 14 -18 carbons, including palmitoyl-CoA, S-palmitoyl-N-acetylcysteamine, and palmitoylated proteins. In contrast to PPT2, PPT1 can hydrolyze palmitoylated proteins and palmitoylcysteine.

Biological Context

Subcellular Location: Lysosome; Secreted; Golgi apparatus; Endoplasmic reticulum
Tissue Specificity: Spleen, brain, seminal vesicle, and testis. Lower levels of activity in liver, heart, lung, and skeletal muscle

Product Specifications

Recombinant Bovine Palmitoyl-protein thioesterase 1 (PPT1) is a recombinant protein from Bos taurus (Bovine), expressed in E.coli, covering amino acids 28-306aa, with N-terminal 10xHis-tagged and C-terminal Myc-tagged tag, molecular weight 38.9kDa, purity Greater than 85% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

Related products

Protein Biology

Function

Has thioesterase activity against fatty acid thioesters with 14 -18 carbons, including palmitoyl-CoA, S-palmitoyl-N-acetylcysteamine, and palmitoylated proteins (PubMed:7916016). In contrast to PPT2, PPT1 can hydrolyze palmitoylated proteins and palmitoylcysteine (By similarity)

Subcellular Location

Lysosome; Secreted; Golgi apparatus; Endoplasmic reticulum

Tissue Specificity

Spleen, brain, seminal vesicle, and testis. Lower levels of activity in liver, heart, lung, and skeletal muscle

Subunit

Interacts with CLN5, ATP5F1A and ATP5F1B

Gene: PPT1  |  Organism: Bos taurus  |  Synonyms: Palmitoyl-protein hydrolase 1

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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