Recombinant Bovine respiratory syncytial virus Nucleoprotein (N), Bovine respiratory syncytial virus (strain 391-2) (BRS), E.coli, N-terminal 6xHis-SUMO-tagged, 59.6kDa, purity >90% as determined by SDS-PAGE.
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Recombinant Bovine respiratory syncytial virus Nucleoprotein (N)

Recombinant Bovine respiratory syncytial virus Nucleoprotein (N) — Encapsidates the viral RNA genome by forming a left-handed helical nucleocapsid that protects the RNA from nucleases. Purity >90%.

SKU: BCRECP-003482 Categories: ,
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Product Specifications

Product SkuBCRECP-003482
Product DescriptionRecombinant Bovine respiratory syncytial virus Nucleoprotein (N) Protein is expressed from E.coli with N-terminal 6xHis-SUMO-tagged. It contains 1-391aa. [Accession | P35943].
Uniprot No.P35943
Gene NamesN
PurityGreater than 90% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region1-391aa
SpeciesBovine respiratory syncytial virus (strain 391-2) (BRS)
Tag InfoN-terminal 6xHis-SUMO-tagged
Molecular weight59.6kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasOthers

Function

Encapsidates the viral RNA genome by forming a left-handed helical nucleocapsid that protects the RNA from nucleases. RNA replication depends on the availability of soluble nucleoprotein.

Product Specifications

Recombinant Bovine respiratory syncytial virus Nucleoprotein (N) — Encapsidates the viral RNA genome by forming a left-handed helical nucleocapsid that protects the RNA from nucleases. Purity >90%. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

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Protein Biology

Function

Encapsidates the viral RNA genome by forming a left-handed helical nucleocapsid that protects the RNA from nucleases. RNA replication depends on the availability of soluble nucleoprotein. The encapsidated genomic RNA is termed the NC and serves as template for transcription and replication. Together with the phosphoprotein, sequesters host NF-kappa-B in inclusion bodies (IBs) thereby inhibiting this host defense pathway. May also act as a modulator of the innate immune response by sequestration of host IFIH1/MDA5 and MAVS into IBs

Subcellular Location

Virion; Host cytoplasm

Subunit

Homomultimerizes to form the nucleocapsid. Binds to viral genomic RNA. Interacts with the phosphoprotein P. When in a monomeric RNA-free form, interacts with the phosphoprotein (via N-terminus). Interacts with protein M2-1; this interaction allows the association of nucleocapsid with the matrix protein. Interacts with host EIF2AK2/PKR; this interaction inhibits EIF2AK2 phosphorylation of EIF2S1 and blocks EIF2AK2-mediated translation shutoff. Interacts with host EIF1AX; this interaction recruits EIF1AX to the viral replication complex to facilitate viral genomic RNA synthesis and virus production (By similarity). Interacts with host NF-kappa-B; this interaction sequesters NF-kappa-B in inclusion bodies (By similarity)

Gene: N  |  Organism: Bovine respiratory syncytial virus (strain 391-2)  |  Synonyms: Nucleocapsid protein

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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