Recombinant Bovine respiratory syncytial virus Nucleoprotein (N), Bovine respiratory syncytial virus (strain 391-2) (BRS), E.coli, N-terminal 6xHis-SUMO-tagged, 59.6kDa, purity >90% as determined by SDS-PAGE.
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Recombinant Bovine respiratory syncytial virus Nucleoprotein (N)

Recombinant Bovine respiratory syncytial virus Nucleoprotein (N) — Encapsidates the viral RNA genome by forming a left-handed helical nucleocapsid that protects the RNA from nucleases. Purity >90%.

SKU: BCRECP-003482 Categories: ,
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Product Specifications

Product SkuBCRECP-003482
Product DescriptionRecombinant Bovine respiratory syncytial virus Nucleoprotein (N) Protein is expressed from E.coli with N-terminal 6xHis-SUMO-tagged. It contains 1-391aa. [Accession | P35943].
Uniprot No.P35943
Gene NamesN
PurityGreater than 90% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region1-391aa
SpeciesBovine respiratory syncytial virus (strain 391-2) (BRS)
Tag InfoN-terminal 6xHis-SUMO-tagged
Molecular weight59.6kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasOthers

Function

Encapsidates the viral RNA genome by forming a left-handed helical nucleocapsid that protects the RNA from nucleases. RNA replication depends on the availability of soluble nucleoprotein.

Product Specifications

Recombinant Bovine respiratory syncytial virus Nucleoprotein (N) — Encapsidates the viral RNA genome by forming a left-handed helical nucleocapsid that protects the RNA from nucleases. Purity >90%. Suitable for ELISA and Western Blot applications.

Recommended applications depend on the target, product format, and product-specific validation data. Refer to the product datasheet or Certificate of Analysis (CoA), or contact BioCrest Sci for application guidance.

Biological Activity: Please contact us to obtain bioactivity data.

Storage: Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shipping & Lead Time: Availability, shipping conditions, and estimated lead time are confirmed in the formal quotation or order confirmation based on the product format, destination, and order requirements.

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Protein Biology

Function

Encapsidates the viral RNA genome by forming a left-handed helical nucleocapsid that protects the RNA from nucleases. RNA replication depends on the availability of soluble nucleoprotein. The encapsidated genomic RNA is termed the NC and serves as template for transcription and replication. Together with the phosphoprotein, sequesters host NF-kappa-B in inclusion bodies (IBs) thereby inhibiting this host defense pathway. May also act as a modulator of the innate immune response by sequestration of host IFIH1/MDA5 and MAVS into IBs

Subcellular Location

Virion; Host cytoplasm

Subunit

Homomultimerizes to form the nucleocapsid. Binds to viral genomic RNA. Interacts with the phosphoprotein P. When in a monomeric RNA-free form, interacts with the phosphoprotein (via N-terminus). Interacts with protein M2-1; this interaction allows the association of nucleocapsid with the matrix protein. Interacts with host EIF2AK2/PKR; this interaction inhibits EIF2AK2 phosphorylation of EIF2S1 and blocks EIF2AK2-mediated translation shutoff. Interacts with host EIF1AX; this interaction recruits EIF1AX to the viral replication complex to facilitate viral genomic RNA synthesis and virus production (By similarity). Interacts with host NF-kappa-B; this interaction sequesters NF-kappa-B in inclusion bodies (By similarity)

Gene: N  |  Organism: Bovine respiratory syncytial virus (strain 391-2)  |  Synonyms: Nucleocapsid protein

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How do I order or inquire about this product?

Submit the Online Inquiry form with your required quantity and specifications, or email sales@biocrestsci.com. Available sizes, pricing, stock status, documentation, and estimated lead time are confirmed in the formal quotation.

What is the shipping and delivery time?

Availability, dispatch timing, shipping conditions, and delivery estimates depend on the product format, destination, and order requirements. These details are confirmed in the formal quotation or order confirmation.

How should this product be stored?

Follow the storage and handling instructions in the product-specific datasheet and Certificate of Analysis (CoA). Requirements may vary by product form and formulation; contact BioCrest Sci if product-specific guidance is needed.

What quality controls are performed on your products?

Quality-control data such as identity, purity, endotoxin level, or biological activity are provided only when specified on this product page, the datasheet, or Certificate of Analysis (CoA). Biological activity is not implied unless explicitly stated.

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