Recombinant Bovine Sorting nexin-5 (SNX5), Bos taurus (Bovine), E.coli, C-terminal 6xHis-tagged, 53.6kDa, purity >95% as determined by SDS-PAGE.

Recombinant Bovine Sorting nexin-5 (SNX5)

Recombinant Bovine Sorting nexin-5 (SNX5) — Involved in several stages of intracellular trafficking. Purity >95%.

SKU: BCRECP-004438 Category:
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Product Specifications

Product SkuBCRECP-004438
Product DescriptionRecombinant Bovine Sorting nexin-5 (SNX5) Protein is expressed from E.coli with C-terminal 6xHis-tagged. It contains 2-404aa. [Accession | Q3ZBM5].
Uniprot No.Q3ZBM5
Gene NamesSNX5
PurityGreater than 95% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region2-404aa
SpeciesBos taurus (Bovine)
Tag InfoC-terminal 6xHis-tagged
Molecular weight53.6kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasSignal Transduction

Function

Involved in several stages of intracellular trafficking. Interacts with membranes containing phosphatidylinositol lipids. Acts in part as component of the retromer membrane-deforming SNX-BAR subcomplex.

Biological Context

Subcellular Location: Endosome; Early endosome; Early endosome membrane (Peripheral membrane protein); Cell membrane (Peripheral membrane protein); Cytoplasmic vesicle membrane (Peripheral membrane protein); Cytoplasm; Cell projection, phagocytic cup; Cell projection, ruffle

Product Specifications

Recombinant Bovine Sorting nexin-5 (SNX5) is a recombinant protein from Bos taurus (Bovine), expressed in E.coli, covering amino acids 2-404aa, with C-terminal 6xHis-tagged tag, molecular weight 53.6kDa, purity Greater than 95% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

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Protein Biology

Function

Involved in several stages of intracellular trafficking. Interacts with membranes containing phosphatidylinositol lipids. Acts in part as component of the retromer membrane-deforming SNX-BAR subcomplex. The SNX-BAR retromer mediates retrograde transport of cargo proteins from endosomes to the trans-Golgi network (TGN) and is involved in endosome-to-plasma membrane transport for cargo protein recycling. The SNX-BAR subcomplex functions to deform the donor membrane into a tubular profile called endosome-to-TGN transport carrier (ETC). Does not have in vitro vesicle-to-membrane remodeling activity. Involved in retrograde transport of lysosomal enzyme receptor IGF2R. May function as link between endosomal transport vesicles and dynactin. Plays a role in the internalization of EGFR after EGF stimulation. Involved in EGFR endosomal sorting and degradation; the function involves PIP5K1C and is retromer-independent. Together with PIP5K1C facilitates HGS interaction with ubiquitinated EGFR, which initiates EGFR sorting to intraluminal vesicles (ILVs) of the multivesicular body for subsequent lysosomal degradation. Involved in E-cadherin sorting and degradation; inhibits PIP5K1C-mediated E-cadherin degradation. Plays a role in macropinocytosis (By similarity)

Subcellular Location

Endosome; Early endosome; Early endosome membrane (Peripheral membrane protein); Cell membrane (Peripheral membrane protein); Cytoplasmic vesicle membrane (Peripheral membrane protein); Cytoplasm; Cell projection, phagocytic cup; Cell projection, ruffle

Subunit

Forms heterodimers with BAR domain-containing sorting nexins SNX1 and SNX2; does not homodimerize (By similarity). Interacts with SNX32 (By similarity). The heterodimers are proposed to self-assemble into helical arrays on the membrane to stabilize and expand local membrane curvature underlying endosomal tubule formation. Thought to be a component of the originally described retromer complex (also called SNX-BAR retromer) which is a pentamer containing the heterotrimeric retromer cargo-selective complex (CSC), also described as vacuolar protein sorting subcomplex (VPS), and a heterodimeric membrane-deforming subcomplex formed between SNX1 or SNX2 and SNX5 or SNX6 (also called SNX-BAR subcomplex); the respective CSC and SNX-BAR subcomplexes associate with low affinity. Interacts with SNX1, SNX2, VPS26A, VPS29, VPS35, DCTN1, DOCK1, MIB1, PIP5K1C. Interacts with HGS; increased by PIP5K1C kinase activity and by PtdIns(3P) and/or PtdIns(3,4)P2 (By similarity)

Gene: SNX5  |  Organism: Bos taurus

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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