Recombinant Caenorhabditis elegans Dauer larva development regulatory growth factor daf-7 (daf-7), Caenorhabditis elegans, E.coli, N-terminal 10xHis-tagged and C-terminal Myc-tagged, 18.1kDa, purity >90% as determined by SDS-PAGE.
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Recombinant Caenorhabditis elegans Dauer larva development regulatory growth factor daf-7 (daf-7)

Recombinant Caenorhabditis elegans Dauer larva development regulatory growth factor daf-7 (daf-7) — Under harsh environmental conditions, larvae enter a developmentally arrested state known as dauer; TGF-beta-like daf-7 acts to inhibit dauer larva formation and promote growth. Purity >90%.

SKU: BCRECP-002503 Categories: ,
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Product Specifications

Product SkuBCRECP-002503
Product DescriptionRecombinant Caenorhabditis elegans Dauer larva development regulatory growth factor daf-7 (daf-7) Protein is expressed from E.coli with N-terminal 10xHis-tagged and C-terminal Myc-tagged. It contains 235-350aa. [Accession | P92172].
Uniprot No.P92172
Gene Namesdaf-7
PurityGreater than 90% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region235-350aa
SpeciesCaenorhabditis elegans
Tag InfoN-terminal 10xHis-tagged and C-terminal Myc-tagged
Molecular weight18.1kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasOthers

Function

Under harsh environmental conditions, larvae enter a developmentally arrested state known as dauer; TGF-beta-like daf-7 acts to inhibit dauer larva formation and promote growth. May be a ligand to cell surface receptor daf-4. May act as a negative regulator of dauer larva development by transducing chemosensory information from ASI neurons.

Biological Context

Subcellular Location: Secreted
Tissue Specificity: Expressed in the chemosensory neurons, including in the ASJ neurons in males. Expressed in the ASI neurons

Product Specifications

Recombinant Caenorhabditis elegans Dauer larva development regulatory growth factor daf-7 (daf-7) is a recombinant protein from Caenorhabditis elegans, expressed in E.coli, covering amino acids 235-350aa, with N-terminal 10xHis-tagged and C-terminal Myc-tagged tag, molecular weight 18.1kDa, purity Greater than 90% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

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Protein Biology

Function

Under harsh environmental conditions, larvae enter a developmentally arrested state known as dauer; TGF-beta-like daf-7 acts to inhibit dauer larva formation and promote growth (PubMed:37060828, PubMed:8910282). May be a ligand to cell surface receptor daf-4 (PubMed:8413626). May act as a negative regulator of dauer larva development by transducing chemosensory information from ASI neurons (PubMed:8910282). Involved in sensitivity to CO2 levels (PubMed:18524955). Involved in mate searching behavior of males, acting in concert with the neuropeptide pdf-1 (PubMed:30024377). In AWC neurons, acts to promote expression of srsx-3, a member of the GPCR family (PubMed:20713521)

Subcellular Location

Secreted

Tissue Specificity

Expressed in the chemosensory neurons, including in the ASJ neurons in males. Expressed in the ASI neurons (PubMed:37060828)

Gene: daf-7  |  Organism: Caenorhabditis elegans  |  Synonyms: Abnormal dauer formation protein 7
Key Publications

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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