Recombinant Calloselasma rhodostoma Snaclec rhodocytin subunit beta, Calloselasma rhodostoma (Malayan pit viper) (Agkistrodon rhodostoma), E.coli, N-terminal 10xHis-tagged and C-terminal Myc-tagged, 21.8kDa, purity >85% as determined by SDS-PAGE.

Recombinant Calloselasma rhodostoma Snaclec rhodocytin subunit beta

Recombinant Calloselasma rhodostoma Snaclec rhodocytin subunit beta — Elicits platelet aggregation by the binding to the C-type lectin domain family 1 member B (CLEC1B/CLEC2). Purity >85%.

SKU: BCRECP-003764 Category:
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Product Specifications

Product SkuBCRECP-003764
Product DescriptionRecombinant Calloselasma rhodostoma Snaclec rhodocytin subunit beta Protein is expressed from E.coli with N-terminal 10xHis-tagged and C-terminal Myc-tagged. It contains 24-146aa. [Accession | Q9I840].
Uniprot No.Q9I840
PurityGreater than 85% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region24-146aa
SpeciesCalloselasma rhodostoma (Malayan pit viper) (Agkistrodon rhodostoma)
Tag InfoN-terminal 10xHis-tagged and C-terminal Myc-tagged
Molecular weight21.8kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasOthers

Function

Elicits platelet aggregation by the binding to the C-type lectin domain family 1 member B (CLEC1B/CLEC2). Binding leads to tyrosine phosphorylation in the cytoplasmic tail of CLEC1B, which promotes the binding of spleen tyrosine kinase (Syk), subsequent activation of PLCgamma2, and platelet activation and aggregation. Binding to GPIbalpha (GP1BA) and alpha2/beta-1 (ITGA2/ITGB1) may also induce aggregation, but this is controversial.

Biological Context

Subcellular Location: Secreted
Tissue Specificity: Expressed by the venom gland

Product Specifications

Recombinant Calloselasma rhodostoma Snaclec rhodocytin subunit beta is a recombinant protein from Calloselasma rhodostoma (Malayan pit viper) (Agkistrodon rhodostoma), expressed in E.coli, covering amino acids 24-146aa, with N-terminal 10xHis-tagged and C-terminal Myc-tagged tag, molecular weight 21.8kDa, purity Greater than 85% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

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Protein Biology

Function

Elicits platelet aggregation by the binding to the C-type lectin domain family 1 member B (CLEC1B/CLEC2). Binding leads to tyrosine phosphorylation in the cytoplasmic tail of CLEC1B, which promotes the binding of spleen tyrosine kinase (Syk), subsequent activation of PLCgamma2, and platelet activation and aggregation. Binding to GPIbalpha (GP1BA) and alpha2/beta-1 (ITGA2/ITGB1) may also induce aggregation, but this is controversial

Subcellular Location

Secreted

Tissue Specificity

Expressed by the venom gland

Subunit

Dimer (non-covalently linked) of heterodimers of subunits alpha and beta (disulfide-linked)

Organism: Calloselasma rhodostoma  |  Synonyms: Aggretin beta chain; Rhodoaggretin subunit beta
Key Publications

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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