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Recombinant Candida albicans Secreted aspartic protease 6 (SAP6)

Recombinant Candida albicans Secreted aspartic protease 6 (SAP6) — Secreted aspartic peptidases (SAPs) are a group of ten acidic hydrolases considered as key virulence factors. Purity >85%.

SKU: BCRECP-004372 Categories: , ,

Product Specifications

Product SkuBCRECP-004372
Product DescriptionRecombinant Candida albicans Secreted aspartic protease 6 (SAP6) Protein is expressed from E.coli with N-terminal 6xHis-tagged. It contains 77-418aa. [Accession | P43095].
Uniprot No.P43095
Gene NamesSAP6
PurityGreater than 85% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region77-418aa
SpeciesCandida albicans (Yeast)
Tag InfoN-terminal 6xHis-tagged
Molecular weight39.8kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasOthers

Function

Secreted aspartic peptidases (SAPs) are a group of ten acidic hydrolases considered as key virulence factors. These enzymes supply the fungus with nutrient amino acids as well as are able to degrade the selected host's proteins involved in the immune defense. Moreover, acts toward human hemoglobin though limited proteolysis to generate a variety of antimicrobial hemocidins, enabling to compete with the other microorganisms of the same physiological niche using the microbicidal peptides generated from the host protein.

Biological Context

Subcellular Location: Secreted

Product Specifications

Recombinant Candida albicans Secreted aspartic protease 6 (SAP6) is a recombinant protein from Candida albicans (Yeast), expressed in E.coli, covering amino acids 77-418aa, with N-terminal 6xHis-tagged tag, molecular weight 39.8kDa, purity Greater than 85% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

Protein Biology

Function

Secreted aspartic peptidases (SAPs) are a group of ten acidic hydrolases considered as key virulence factors (PubMed:11478679, PubMed:12065511, PubMed:17652724, PubMed:20713630, PubMed:21540243, PubMed:22302440). These enzymes supply the fungus with nutrient amino acids as well as are able to degrade the selected host's proteins involved in the immune defense (PubMed:11478679, PubMed:12065511, PubMed:17652724, PubMed:20713630, PubMed:21540243, PubMed:22302440). Moreover, acts toward human hemoglobin though limited proteolysis to generate a variety of antimicrobial hemocidins, enabling to compete with the other microorganisms of the same physiological niche using the microbicidal peptides generated from the host protein (PubMed:23927842)

Subcellular Location

Secreted

Gene: SAP6  |  Organism: Candida albicans  |  Synonyms: Candidapepsin-6
Key Publications

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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