Recombinant Chicken Indian hedgehog protein (IHH), Gallus gallus (Chicken), E.coli, N-terminal 10xHis-tagged and C-terminal Myc-tagged, 47.5kDa, purity >90% as determined by SDS-PAGE.

Recombinant Chicken Indian hedgehog protein (IHH)

Recombinant Chicken Indian hedgehog protein (IHH) — The dually lipidated indian hedgehog protein N-product is a morphogen that activates the smoothened signaling pathway, and which is essential for bone and cartilage development. Purity >90%.

SKU: BCRECP-003099 Category:
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Product Specifications

Product SkuBCRECP-003099
Product DescriptionRecombinant Chicken Indian hedgehog protein (IHH) Protein is expressed from E.coli with N-terminal 10xHis-tagged and C-terminal Myc-tagged. It contains 24-408aa. [Accession | Q98938].
Uniprot No.Q98938
Gene NamesIHH
PurityGreater than 90% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region24-408aa
SpeciesGallus gallus (Chicken)
Tag InfoN-terminal 10xHis-tagged and C-terminal Myc-tagged
Molecular weight47.5kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasOthers

Function

The dually lipidated indian hedgehog protein N-product is a morphogen that activates the smoothened signaling pathway, and which is essential for bone and cartilage development. Acts by binding to the patched receptor (PTCH1 or PTCH2), relieving smoothened (SMO) inhibition by patched, activating the smoothened signaling pathway and transcription of target genes. In the absence of IHH, patched represses the constitutive signaling activity of SMO.

Biological Context

Subcellular Location: Secreted; Cell membrane (Lipid-anchor)
Tissue Specificity: Expressed in developing midgut, lung and cartilage of developing long bones in the limb

Product Specifications

Recombinant Chicken Indian hedgehog protein (IHH) is a recombinant protein from Gallus gallus (Chicken), expressed in E.coli, covering amino acids 24-408aa, with N-terminal 10xHis-tagged and C-terminal Myc-tagged tag, molecular weight 47.5kDa, purity Greater than 90% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

Related products

Protein Biology

Function

The dually lipidated indian hedgehog protein N-product is a morphogen that activates the smoothened signaling pathway, and which is essential for bone and cartilage development (PubMed:8662546). Acts by binding to the patched receptor (PTCH1 or PTCH2), relieving smoothened (SMO) inhibition by patched, activating the smoothened signaling pathway and transcription of target genes (By similarity). In the absence of IHH, patched represses the constitutive signaling activity of SMO (By similarity). Plays a role in morphogenesis of the skeleton by coordinating growth and differentiation of the endochondral skeleton (PubMed:8662546). Positively regulates PTHLH expression during endochondral bone formation preventing chondrocyte hypertrophy (PubMed:8662546). In contrast, participates in normal chondrocyte proliferation in a PTHLH-independent pathway (PubMed:8662546)

Subcellular Location

Secreted; Cell membrane (Lipid-anchor)

Tissue Specificity

Expressed in developing midgut, lung and cartilage of developing long bones in the limb

Subunit

Interacts with BOC and CDON. Interacts with PTCH1 (By similarity). Interacts with glypican GPC3 (By similarity)

Gene: IHH  |  Organism: Gallus gallus

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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