Recombinant Chikungunya virus Non-structural protein 4 (nsP4), partial, Chikungunya virus (strain S27-African prototype) (CHIKV), E.coli, N-terminal 6xHis-tagged, 31.1kDa, purity >90% as determined by SDS-PAGE.
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Recombinant Chikungunya virus Non-structural protein 4 (nsP4), partial

Recombinant Chikungunya virus Non-structural protein 4 (nsP4), partial — RNA dependent RNA polymerase. Purity >90%.

SKU: BCRECP-003913 Categories: ,
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Product Specifications

Product SkuBCRECP-003913
Product DescriptionRecombinant Chikungunya virus Non-structural protein 4 (nsP4) Protein is expressed from E.coli with N-terminal 6xHis-tagged. It contains 2228-2474aa. [Accession | Q8JUX6].
Uniprot No.Q8JUX6
Gene Namesnsp4
PurityGreater than 90% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region2228-2474aa
SpeciesChikungunya virus (strain S27-African prototype) (CHIKV)
Tag InfoN-terminal 6xHis-tagged
Molecular weight31.1kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasOthers

Function

RNA dependent RNA polymerase. Replicates genomic and antigenomic RNA by recognizing replications specific signals. The early replication complex formed by the polyprotein P123 and nsP4 synthesizes minus-strand RNAs.

Biological Context

Subcellular Location: Host cytoplasmic vesicle membrane (Peripheral membrane protein)

Product Specifications

Recombinant Chikungunya virus Non-structural protein 4 (nsP4), partial is a recombinant protein from Chikungunya virus (strain S27-African prototype) (CHIKV), expressed in E.coli, covering amino acids 2228-2474aa, with N-terminal 6xHis-tagged tag, molecular weight 31.1kDa, purity Greater than 90% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

Recommended applications depend on the target, product format, and product-specific validation data. Refer to the product datasheet or Certificate of Analysis (CoA), or contact BioCrest Sci for application guidance.

Biological Activity: Please contact us to obtain bioactivity data.

Storage: Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shipping & Lead Time: Availability, shipping conditions, and estimated lead time are confirmed in the formal quotation or order confirmation based on the product format, destination, and order requirements.

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Protein Biology

Function

RNA dependent RNA polymerase (By similarity). Replicates genomic and antigenomic RNA by recognizing replications specific signals. The early replication complex formed by the polyprotein P123 and nsP4 synthesizes minus-strand RNAs (By similarity). The late replication complex composed of fully processed nsP1-nsP4 is responsible for the production of genomic and subgenomic plus-strand RNAs (By similarity)

Subcellular Location

Host cytoplasmic vesicle membrane (Peripheral membrane protein)

Subunit

Interacts with RNA-directed RNA polymerase nsP4 (PubMed:22951312). Interacts with mRNA-capping enzyme nsP1 (PubMed:22951312). Interacts with KPNA1/karyopherin-alpha1; this interaction probably allows the active transport of protease nsP2 into the host nucleus (By similarity)

Organism: Chikungunya virus (strain S27-African prototype)  |  Synonyms: Non-structural polyprotein
Key Publications

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Frequently Asked Questions

How do I order or inquire about this product?

Submit the Online Inquiry form with your required quantity and specifications, or email sales@biocrestsci.com. Available sizes, pricing, stock status, documentation, and estimated lead time are confirmed in the formal quotation.

What is the shipping and delivery time?

Availability, dispatch timing, shipping conditions, and delivery estimates depend on the product format, destination, and order requirements. These details are confirmed in the formal quotation or order confirmation.

How should this product be stored?

Follow the storage and handling instructions in the product-specific datasheet and Certificate of Analysis (CoA). Requirements may vary by product form and formulation; contact BioCrest Sci if product-specific guidance is needed.

What quality controls are performed on your products?

Quality-control data such as identity, purity, endotoxin level, or biological activity are provided only when specified on this product page, the datasheet, or Certificate of Analysis (CoA). Biological activity is not implied unless explicitly stated.

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