Recombinant Chlamydia trachomatis serovar D Large cysteine-rich periplasmic protein OmcB (omcB), partial, Chlamydia trachomatis (strain D/UW-3/Cx), E.coli, N-terminal 6xHis-SUMO-tagged, 33.1kDa, purity >90% as determined by SDS-PAGE.

Recombinant Chlamydia trachomatis serovar D Large cysteine-rich periplasmic protein OmcB (omcB), partial

Recombinant Chlamydia trachomatis serovar D Large cysteine-rich periplasmic protein OmcB (omcB), partial — In elementary bodies (EBs, the infectious stage, which is able to survive outside the host cell) provides the structural integrity of the outer envelope through disulfide cross-links with th…

SKU: BCRECP-003934 Category:
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Product Specifications

Product SkuBCRECP-003934
Product DescriptionRecombinant Chlamydia trachomatis serovar D Large cysteine-rich periplasmic protein OmcB (omcB) Protein is expressed from E.coli with N-terminal 6xHis-SUMO-tagged. It contains 41-196aa. [Accession | P0CC04].
Uniprot No.P0CC04
Gene NamesomcB
PurityGreater than 90% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region41-196aa
SpeciesChlamydia trachomatis (strain D/UW-3/Cx)
Tag InfoN-terminal 6xHis-SUMO-tagged
Molecular weight33.1kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasMicrobiology

Function

In elementary bodies (EBs, the infectious stage, which is able to survive outside the host cell) provides the structural integrity of the outer envelope through disulfide cross-links with the small cysteine-rich protein and the major outer membrane protein. It has been described in publications as the Sarkosyl-insoluble COMC (Chlamydia outer membrane complex), and serves as the functional equivalent of peptidoglycan.

Biological Context

Subcellular Location: Periplasm

Product Specifications

Recombinant Chlamydia trachomatis serovar D Large cysteine-rich periplasmic protein OmcB (omcB), partial is a recombinant protein from Chlamydia trachomatis (strain D/UW-3/Cx), expressed in E.coli, covering amino acids 41-196aa, with N-terminal 6xHis-SUMO-tagged tag, molecular weight 33.1kDa, purity Greater than 90% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

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Protein Biology

Function

In elementary bodies (EBs, the infectious stage, which is able to survive outside the host cell) provides the structural integrity of the outer envelope through disulfide cross-links with the small cysteine-rich protein and the major outer membrane protein. It has been described in publications as the Sarkosyl-insoluble COMC (Chlamydia outer membrane complex), and serves as the functional equivalent of peptidoglycan (By similarity)

Subcellular Location

Periplasm

Subunit

Part of a disulfide cross-linked outer membrane complex (COMC) composed of the major outer membrane porin (MOMP), the small cysteine-rich protein (OmcA) and the large cysteine-rich periplasmic protein (OmcB)

Gene: omcB  |  Organism: Chlamydia trachomatis serovar D (strain ATCC VR-885 / DSM 19411 / UW-3/Cx)  |  Synonyms: 60 kDa cysteine-rich OMP; 60 kDa outer membrane protein; Cysteine-rich outer membrane protein

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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