Recombinant Clostridium acetobutylicum Butyrate--acetoacetate CoA-transferase subunit B (ctfB), Clostridium acetobutylicum (strain ATCC 824 / DSM 792 / JCM 1419 / LMG 5710 / VKM B-1787), E.coli, N-terminal 10xHis-tagged and C-terminal Myc-tagged, 30.6kDa, purity >85% as determined by SDS-PAGE.
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Recombinant Clostridium acetobutylicum Butyrate–acetoacetate CoA-transferase subunit B (ctfB)

Recombinant Clostridium acetobutylicum Butyrate–acetoacetate CoA-transferase subunit B (ctfB) — Catalyzes the transfer of CoA from acetoacetyl-CoA to acetate, butyrate and propionate. Purity >85%.

SKU: BCRECP-002470 Categories: ,
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Product Specifications

Product SkuBCRECP-002470
Product DescriptionRecombinant Clostridium acetobutylicum Butyrate--acetoacetate CoA-transferase subunit B (ctfB) Protein is expressed from E.coli with N-terminal 10xHis-tagged and C-terminal Myc-tagged. It contains 1-221aa. [Accession | P23673].
Uniprot No.P23673
Gene NamesctfB
PurityGreater than 85% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region1-221aa
SpeciesClostridium acetobutylicum (strain ATCC 824 / DSM 792 / JCM 1419 / LMG 5710 / VKM B-1787)
Tag InfoN-terminal 10xHis-tagged and C-terminal Myc-tagged
Molecular weight30.6kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasEpigenetics and Nuclear Signaling

Function

Catalyzes the transfer of CoA from acetoacetyl-CoA to acetate, butyrate and propionate. Also shows low activity with valerate, isobutyrate and crotonate. Plays an important role in the metabolic shift between the acid-producing and solvent-forming states of C.acetobutylicum.

Product Specifications

Recombinant Clostridium acetobutylicum Butyrate–acetoacetate CoA-transferase subunit B (ctfB) is a recombinant protein from Clostridium acetobutylicum (strain ATCC 824 / DSM 792 / JCM 1419 / LMG 5710 / VKM B-1787), expressed in E.coli, covering amino acids 1-221aa, with N-terminal 10xHis-tagged and C-terminal Myc-tagged tag, molecular weight 30.6kDa, purity Greater than 85% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

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Protein Biology

Function

Catalyzes the transfer of CoA from acetoacetyl-CoA to acetate, butyrate and propionate (PubMed:2383002, PubMed:2719476). Also shows low activity with valerate, isobutyrate and crotonate (PubMed:2719476). Plays an important role in the metabolic shift between the acid-producing and solvent-forming states of C.acetobutylicum (PubMed:2383002). Acts mainly to detoxify the medium by removing the acetate and butyrate excreted earlier in the fermentation (PubMed:2383002, PubMed:2719476)

Subunit

Heterotetramer composed of two alpha subunits (CtfA) and two beta subunits (CtfB)

Gene: ctfB  |  Organism: Clostridium acetobutylicum (strain ATCC 824 / DSM 792 / JCM 1419 / IAM 19013 / LMG 5710 / NBRC 13948 / NRRL B-527 / VKM B-1787 / 2291 / W)  |  Synonyms: Butyrate--acetoacetate CoA-transferase subunit B
Key Publications

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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