Recombinant Clostridium acetobutylicum Butyrate--acetoacetate CoA-transferase subunit B (ctfB), Clostridium acetobutylicum (strain ATCC 824 / DSM 792 / JCM 1419 / LMG 5710 / VKM B-1787), E.coli, N-terminal 10xHis-tagged and C-terminal Myc-tagged, 30.6kDa, purity >85% as determined by SDS-PAGE.
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Recombinant Clostridium acetobutylicum Butyrate–acetoacetate CoA-transferase subunit B (ctfB)

Recombinant Clostridium acetobutylicum Butyrate–acetoacetate CoA-transferase subunit B (ctfB) — Catalyzes the transfer of CoA from acetoacetyl-CoA to acetate, butyrate and propionate. Purity >85%.

SKU: BCRECP-002470 Categories: ,
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Product Specifications

Product SkuBCRECP-002470
Product DescriptionRecombinant Clostridium acetobutylicum Butyrate--acetoacetate CoA-transferase subunit B (ctfB) Protein is expressed from E.coli with N-terminal 10xHis-tagged and C-terminal Myc-tagged. It contains 1-221aa. [Accession | P23673].
Uniprot No.P23673
Gene NamesctfB
PurityGreater than 85% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region1-221aa
SpeciesClostridium acetobutylicum (strain ATCC 824 / DSM 792 / JCM 1419 / LMG 5710 / VKM B-1787)
Tag InfoN-terminal 10xHis-tagged and C-terminal Myc-tagged
Molecular weight30.6kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasEpigenetics and Nuclear Signaling

Function

Catalyzes the transfer of CoA from acetoacetyl-CoA to acetate, butyrate and propionate. Also shows low activity with valerate, isobutyrate and crotonate. Plays an important role in the metabolic shift between the acid-producing and solvent-forming states of C.acetobutylicum.

Product Specifications

Recombinant Clostridium acetobutylicum Butyrate–acetoacetate CoA-transferase subunit B (ctfB) is a recombinant protein from Clostridium acetobutylicum (strain ATCC 824 / DSM 792 / JCM 1419 / LMG 5710 / VKM B-1787), expressed in E.coli, covering amino acids 1-221aa, with N-terminal 10xHis-tagged and C-terminal Myc-tagged tag, molecular weight 30.6kDa, purity Greater than 85% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

Recommended applications depend on the target, product format, and product-specific validation data. Refer to the product datasheet or Certificate of Analysis (CoA), or contact BioCrest Sci for application guidance.

Biological Activity: Please contact us to obtain bioactivity data.

Storage: Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shipping & Lead Time: Availability, shipping conditions, and estimated lead time are confirmed in the formal quotation or order confirmation based on the product format, destination, and order requirements.

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Protein Biology

Function

Catalyzes the transfer of CoA from acetoacetyl-CoA to acetate, butyrate and propionate (PubMed:2383002, PubMed:2719476). Also shows low activity with valerate, isobutyrate and crotonate (PubMed:2719476). Plays an important role in the metabolic shift between the acid-producing and solvent-forming states of C.acetobutylicum (PubMed:2383002). Acts mainly to detoxify the medium by removing the acetate and butyrate excreted earlier in the fermentation (PubMed:2383002, PubMed:2719476)

Subunit

Heterotetramer composed of two alpha subunits (CtfA) and two beta subunits (CtfB)

Gene: ctfB  |  Organism: Clostridium acetobutylicum (strain ATCC 824 / DSM 792 / JCM 1419 / IAM 19013 / LMG 5710 / NBRC 13948 / NRRL B-527 / VKM B-1787 / 2291 / W)  |  Synonyms: Butyrate--acetoacetate CoA-transferase subunit B
Key Publications

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Frequently Asked Questions

How do I order or inquire about this product?

Submit the Online Inquiry form with your required quantity and specifications, or email sales@biocrestsci.com. Available sizes, pricing, stock status, documentation, and estimated lead time are confirmed in the formal quotation.

What is the shipping and delivery time?

Availability, dispatch timing, shipping conditions, and delivery estimates depend on the product format, destination, and order requirements. These details are confirmed in the formal quotation or order confirmation.

How should this product be stored?

Follow the storage and handling instructions in the product-specific datasheet and Certificate of Analysis (CoA). Requirements may vary by product form and formulation; contact BioCrest Sci if product-specific guidance is needed.

What quality controls are performed on your products?

Quality-control data such as identity, purity, endotoxin level, or biological activity are provided only when specified on this product page, the datasheet, or Certificate of Analysis (CoA). Biological activity is not implied unless explicitly stated.

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