Recombinant Clostridium kluyveri NAD-dependent 4-hydroxybutyrate dehydrogenase (4hbD), Clostridium kluyveri (strain ATCC 8527 / DSM 555 / NCIMB 10680), E.coli, N-terminal 10xHis-tagged and C-terminal Myc-tagged, 49.2kDa, purity >85% as determined by SDS-PAGE.
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Recombinant Clostridium kluyveri NAD-dependent 4-hydroxybutyrate dehydrogenase (4hbD)

Recombinant Clostridium kluyveri NAD-dependent 4-hydroxybutyrate dehydrogenase (4hbD) — Involved in the anaerobic succinate degradation pathway. Purity >85%.

SKU: BCRECP-001976 Categories: ,
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Product Specifications

Product SkuBCRECP-001976
Product DescriptionRecombinant Clostridium kluyveri NAD-dependent 4-hydroxybutyrate dehydrogenase (4hbD) Protein is expressed from E.coli with N-terminal 10xHis-tagged and C-terminal Myc-tagged. It contains 1-371aa. [Accession | P38945].
Uniprot No.P38945
Gene Names4hbD
PurityGreater than 85% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region1-371aa
SpeciesClostridium kluyveri (strain ATCC 8527 / DSM 555 / NCIMB 10680)
Tag InfoN-terminal 10xHis-tagged and C-terminal Myc-tagged
Molecular weight49.2kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research Areasothers

Function

Involved in the anaerobic succinate degradation pathway. Catalyzes the interconversion of gamma-hydroxybutyrate (GHB) and succinic semialdehyde (SSA).

Product Specifications

Recombinant Clostridium kluyveri NAD-dependent 4-hydroxybutyrate dehydrogenase (4hbD) is a recombinant protein from Clostridium kluyveri (strain ATCC 8527 / DSM 555 / NCIMB 10680), expressed in E.coli, covering amino acids 1-371aa, with N-terminal 10xHis-tagged and C-terminal Myc-tagged tag, molecular weight 49.2kDa, purity Greater than 85% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

Recommended applications depend on the target, product format, and product-specific validation data. Refer to the product datasheet or Certificate of Analysis (CoA), or contact BioCrest Sci for application guidance.

Biological Activity: Please contact us to obtain bioactivity data.

Storage: Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shipping & Lead Time: Availability, shipping conditions, and estimated lead time are confirmed in the formal quotation or order confirmation based on the product format, destination, and order requirements.

Related products

Protein Biology

Function

Involved in the anaerobic succinate degradation pathway (PubMed:8328804, PubMed:8444151, PubMed:8550525). Catalyzes the interconversion of gamma-hydroxybutyrate (GHB) and succinic semialdehyde (SSA) (PubMed:7606170, PubMed:8328804, PubMed:8550525)

Subunit

Homodimer

Gene: 4hbD  |  Organism: Clostridium kluyveri (strain ATCC 8527 / DSM 555 / NBRC 12016 / NCIMB 10680 / K1)  |  Synonyms: Gamma-hydroxybutyrate dehydrogenase; NAD(+)-dependent 4-hydroxybutyrate dehydrogenase

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Availability, dispatch timing, shipping conditions, and delivery estimates depend on the product format, destination, and order requirements. These details are confirmed in the formal quotation or order confirmation.

How should this product be stored?

Follow the storage and handling instructions in the product-specific datasheet and Certificate of Analysis (CoA). Requirements may vary by product form and formulation; contact BioCrest Sci if product-specific guidance is needed.

What quality controls are performed on your products?

Quality-control data such as identity, purity, endotoxin level, or biological activity are provided only when specified on this product page, the datasheet, or Certificate of Analysis (CoA). Biological activity is not implied unless explicitly stated.

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