Recombinant Cocal virus Glycoprotein (G) Protein, Cocal virus (COCV), in vitro E.coli expression system, N-terminal 10xHis-tagged, 57.6kDa, purity >85% as determined by SDS-PAGE.
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Recombinant Cocal virus Glycoprotein (G) Protein

Recombinant Cocal virus Glycoprotein (G) Protein — Attaches the virus to host receptors, inducing clathrin-dependent endocytosis of the virion. Purity >85%.

SKU: BCRECP-00133TM Categories: ,
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Product Specifications

Uniprot No.O56677
Gene NamesG
PurityGreater than 85% as determined by SDS-PAGE.
Expression Systemin vitro E.coli expression system
Expression Region18-512aa
SpeciesCocal virus (COCV)
Tag InfoN-terminal 10xHis-tagged
Molecular weight57.6kDa
ActivityNot Test
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Function

Attaches the virus to host receptors, inducing clathrin-dependent endocytosis of the virion. In the endosome, the acidic pH induces conformational changes in the glycoprotein trimer, which trigger fusion between virus and endosomal membrane.

Biological Context

Subcellular Location: Virion membrane (Single-pass type I membrane protein); Host membrane (Single-pass type I membrane protein)

Product Specifications

Recombinant Cocal virus Glycoprotein (G) Protein is a recombinant protein from Cocal virus (COCV), expressed in in vitro E.coli expression system, covering amino acids 18-512aa, with N-terminal 10xHis-tagged tag, molecular weight 57.6kDa, purity Greater than 85% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

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Protein Biology

Function

Attaches the virus to host receptors, inducing clathrin-dependent endocytosis of the virion. In the endosome, the acidic pH induces conformational changes in the glycoprotein trimer, which trigger fusion between virus and endosomal membrane

Subcellular Location

Virion membrane (Single-pass type I membrane protein); Host membrane (Single-pass type I membrane protein)

Subunit

Homotrimer. Interacts with host LDL at target cell surface

Gene: G  |  Organism: Cocal virus

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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