Recombinant Cricetulus griseus Protein disulfide-isomerase (P4HB), Cricetulus griseus (Chinese hamster) (Cricetulus barabensis griseus), Yeast, N-terminal 6xHis-tagged, 57.0kDa, purity >90% as determined by SDS-PAGE.
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Recombinant Cricetulus griseus Protein disulfide-isomerase (P4HB)

Recombinant Cricetulus griseus Protein disulfide-isomerase (P4HB) — This multifunctional protein catalyzes the formation, breakage and rearrangement of disulfide bonds. Purity >90%.

SKU: BCRECP-004000 Categories: ,
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Product Specifications

Product SkuBCRECP-004000
Product DescriptionRecombinant Cricetulus griseus Protein disulfide-isomerase (P4HB) Protein is expressed from Yeast with N-terminal 6xHis-tagged. It contains 20-509aa. [Accession | Q8R4U2].
Uniprot No.Q8R4U2
Gene NamesP4HB
PurityGreater than 90% as determined by SDS-PAGE.
Expression SystemYeast
Expression Region20-509aa
SpeciesCricetulus griseus (Chinese hamster) (Cricetulus barabensis griseus)
Tag InfoN-terminal 6xHis-tagged
Molecular weight57.0kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasSignal Transduction

Function

This multifunctional protein catalyzes the formation, breakage and rearrangement of disulfide bonds. At the cell surface, seems to act as a reductase that cleaves disulfide bonds of proteins attached to the cell. May therefore cause structural modifications of exofacial proteins.

Biological Context

Subcellular Location: Endoplasmic reticulum; Endoplasmic reticulum lumen; Melanosome; Cell membrane (Peripheral membrane protein)

Product Specifications

Recombinant Cricetulus griseus Protein disulfide-isomerase (P4HB) is a recombinant protein from Cricetulus griseus (Chinese hamster) (Cricetulus barabensis griseus), expressed in Yeast, covering amino acids 20-509aa, with N-terminal 6xHis-tagged tag, molecular weight 57.0kDa, purity Greater than 90% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

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Protein Biology

Function

This multifunctional protein catalyzes the formation, breakage and rearrangement of disulfide bonds. At the cell surface, seems to act as a reductase that cleaves disulfide bonds of proteins attached to the cell. May therefore cause structural modifications of exofacial proteins. Inside the cell, seems to form/rearrange disulfide bonds of nascent proteins. At high concentrations and following phosphorylation by FAM20C, functions as a chaperone that inhibits aggregation of misfolded proteins. At low concentrations, facilitates aggregation (anti-chaperone activity). May be involved with other chaperones in the structural modification of the TG precursor in hormone biogenesis. Also acts as a structural subunit of various enzymes such as prolyl 4-hydroxylase and microsomal triacylglycerol transfer protein MTTP. Receptor for LGALS9; the interaction retains P4HB at the cell surface of Th2 T helper cells, increasing disulfide reductase activity at the plasma membrane, altering the plasma membrane redox state and enhancing cell migration

Subcellular Location

Endoplasmic reticulum; Endoplasmic reticulum lumen; Melanosome; Cell membrane (Peripheral membrane protein)

Subunit

Heterodimer; heterodimerizes with the protein microsomal triglyceride transfer MTTP. Homodimer. Homodimer. Monomers and homotetramers may also occur. Interacts with P4HA2, forming a heterotetramer consisting of 2 alpha subunits (P4HA2) and 2 beta (P4HB), where P4HB plays the role of a structural subunit; this tetramer catalyzes the formation of 4-hydroxyproline in collagen (By similarity). Also constitutes the structural subunit of the microsomal triacylglycerol transfer protein MTTP in mammalian cells. Stabilizes both enzymes and retain them in the ER without contributing to the catalytic activity. Binds UBQLN1. Interacts with ERO1B. Interacts with ILDR2 (By similarity). Interacts with ERN1/IRE1A (via N-terminus); the interaction is enhanced by phosphorylation of P4HB by FAM20C in response to endoplasmic reticulum stress and results in attenuation of ERN1 activity (By similarity)

Gene: P4HB  |  Organism: Cricetulus griseus  |  Synonyms: Prolyl 4-hydroxylase subunit beta; p58

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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