Recombinant Danio rerio Fin bud initiation factor (fibin), Danio rerio (Zebrafish) (Brachydanio rerio), Baculovirus, N-terminal 10xHis-tagged and C-terminal Myc-tagged, 25.8kDa, purity >85% as determined by SDS-PAGE.

Recombinant Danio rerio Fin bud initiation factor (fibin)

Recombinant Danio rerio Fin bud initiation factor (fibin) — Essential for the initiation of pectoral fin bud formation. Purity >85%.

SKU: BCRECP-002737 Category:
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Product Specifications

Product SkuBCRECP-002737
Product DescriptionRecombinant Danio rerio Fin bud initiation factor (fibin) Protein is expressed from Baculovirus with N-terminal 10xHis-tagged and C-terminal Myc-tagged. It contains 24-210aa. [Accession | A1IGX5].
Uniprot No.A1IGX5
Gene Namesfibin
PurityGreater than 85% as determined by SDS-PAGE.
Expression SystemBaculovirus
Expression Region24-210aa
SpeciesDanio rerio (Zebrafish) (Brachydanio rerio)
Tag InfoN-terminal 10xHis-tagged and C-terminal Myc-tagged
Molecular weight25.8kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasCell Biology

Function

Essential for the initiation of pectoral fin bud formation. Potentially acts downstream of retinoic acid and Wnt signaling and is required for tbx5 expression in the lateral plate mesoderm of presumptive pectoral fin bud regions.

Biological Context

Subcellular Location: Endoplasmic reticulum; Secreted; Golgi apparatus

Product Specifications

Recombinant Danio rerio Fin bud initiation factor (fibin) is a recombinant protein from Danio rerio (Zebrafish) (Brachydanio rerio), expressed in Baculovirus, covering amino acids 24-210aa, with N-terminal 10xHis-tagged and C-terminal Myc-tagged tag, molecular weight 25.8kDa, purity Greater than 85% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

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Protein Biology

Function

Essential for the initiation of pectoral fin bud formation. Potentially acts downstream of retinoic acid and Wnt signaling and is required for tbx5 expression in the lateral plate mesoderm of presumptive pectoral fin bud regions

Subcellular Location

Endoplasmic reticulum; Secreted; Golgi apparatus

Subunit

Homodimer; disulfide-linked. Seems to also exist as monomers (By similarity)

Gene: fibin  |  Organism: Danio rerio

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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