Recombinant Dog B-lymphocyte antigen CD20 (MS4A1) Protein, Canis familiaris (Dog) (Canis lupus familiaris), in vitro E.coli expression system, N-terminal 10xHis-tagged, 34.6kDa, purity >85% as determined by SDS-PAGE.
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Recombinant Dog B-lymphocyte antigen CD20 (MS4A1) Protein

Recombinant Dog B-lymphocyte antigen CD20 (MS4A1) Protein — B-lymphocyte-specific membrane protein that plays a role in the regulation of cellular calcium influx necessary for the development, differentiation, and activation of B-lymphocytes. Purity >85%.

SKU: BCRECP-00006TM Categories: ,
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Product Specifications

Uniprot No.Q3C2E2
Gene NamesMS4A1
PurityGreater than 85% as determined by SDS-PAGE.
Expression Systemin vitro E.coli expression system
Expression Region1-297aa
SpeciesCanis familiaris (Dog) (Canis lupus familiaris)
Tag InfoN-terminal 10xHis-tagged
Molecular weight34.6kDa
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Function

B-lymphocyte-specific membrane protein that plays a role in the regulation of cellular calcium influx necessary for the development, differentiation, and activation of B-lymphocytes. Functions as a store-operated calcium (SOC) channel component promoting calcium influx after activation by the B-cell receptor/BCR.

Biological Context

Subcellular Location: Cell membrane (Multi-pass membrane protein); Cell membrane (Lipid-anchor)
Tissue Specificity: Expressed in PBMCs and lymph node from healthy dogs, in B-cells of canine lymphoma, but not in T-cell lymphoma cells and non-T and non-B-cell lymphoma cells

Product Specifications

Recombinant Dog B-lymphocyte antigen CD20 (MS4A1) Protein is a recombinant protein from Canis familiaris (Dog) (Canis lupus familiaris), expressed in in vitro E.coli expression system, covering amino acids 1-297aa, with N-terminal 10xHis-tagged tag, molecular weight 34.6kDa, purity Greater than 85% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

Related products

Protein Biology

Function

B-lymphocyte-specific membrane protein that plays a role in the regulation of cellular calcium influx necessary for the development, differentiation, and activation of B-lymphocytes. Functions as a store-operated calcium (SOC) channel component promoting calcium influx after activation by the B-cell receptor/BCR

Subcellular Location

Cell membrane (Multi-pass membrane protein); Cell membrane (Lipid-anchor)

Tissue Specificity

Expressed in PBMCs and lymph node from healthy dogs, in B-cells of canine lymphoma, but not in T-cell lymphoma cells and non-T and non-B-cell lymphoma cells

Subunit

Forms homotetramers. Interacts with the heavy and light chains of cell surface IgM, the antigen-binding components of the BCR

Gene: MS4A1  |  Organism: Canis lupus familiaris  |  Synonyms: Membrane-spanning 4-domains subfamily A member 1

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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