Recombinant Dog CD274 molecule (CD274), partial, Canis lupus familiaris (Dog) (Canis familiaris), Mammalian cell, C-terminal 10xHis-tagged, 26.2kDa, purity >95% as determined by SDS-PAGE.
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Recombinant Dog CD274 molecule (CD274), partial

Recombinant Dog CD274 molecule (CD274), partial — The PDCD1-mediated inhibitory pathway is exploited by tumors to attenuate anti-tumor immunity and escape destruction by the immune system, thereby facilitating tumor survival. Purity >95%.

SKU: BCRECP-002316 Categories: ,
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Product Specifications

Product SkuBCRECP-002316
Product DescriptionRecombinant Dog CD274 molecule (CD274) Protein is expressed from Mammalian cell with C-terminal 10xHis-tagged. It contains 19-236aa. [Accession | E2RKZ5].
Uniprot No.E2RKZ5
Gene NamesCD274
PurityGreater than 95% as determined by SDS-PAGE.
Expression SystemMammalian cell
Expression Region19-236aa
SpeciesCanis lupus familiaris (Dog) (Canis familiaris)
Tag InfoC-terminal 10xHis-tagged
Molecular weight26.2kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasCancer

Function

The PDCD1-mediated inhibitory pathway is exploited by tumors to attenuate anti-tumor immunity and escape destruction by the immune system, thereby facilitating tumor survival. The interaction with PDCD1/PD-1 inhibits cytotoxic T lymphocytes (CTLs) effector function. The blockage of the PDCD1-mediated pathway results in the reversal of the exhausted T-cell phenotype and the normalization of the anti-tumor response, providing a rationale for cancer immunotherapy.

Biological Context

Subcellular Location: Cell membrane (Single-pass type I membrane protein); Early endosome membrane (Single-pass type I membrane protein); Recycling endosome membrane (Single-pass type I membrane protein)

Product Specifications

Recombinant Dog CD274 molecule (CD274), partial is a recombinant protein from Canis lupus familiaris (Dog) (Canis familiaris), expressed in Mammalian cell, covering amino acids 19-236aa, with C-terminal 10xHis-tagged tag, molecular weight 26.2kDa, purity Greater than 95% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

Related products

Protein Biology

Function

The PDCD1-mediated inhibitory pathway is exploited by tumors to attenuate anti-tumor immunity and escape destruction by the immune system, thereby facilitating tumor survival. The interaction with PDCD1/PD-1 inhibits cytotoxic T lymphocytes (CTLs) effector function. The blockage of the PDCD1-mediated pathway results in the reversal of the exhausted T-cell phenotype and the normalization of the anti-tumor response, providing a rationale for cancer immunotherapy

Subcellular Location

Cell membrane (Single-pass type I membrane protein); Early endosome membrane (Single-pass type I membrane protein); Recycling endosome membrane (Single-pass type I membrane protein)

Gene: PDL1  |  Organism: Canis lupus familiaris  |  Synonyms: B7 homolog 1

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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