Recombinant Dog Glycoprotein hormones alpha chain (CGA), Canis lupus familiaris (Dog) (Canis familiaris), E.coli, N-terminal 10xHis-tagged and C-terminal Myc-tagged, 18.1kDa, purity >90% as determined by SDS-PAGE.

Recombinant Dog Glycoprotein hormones alpha chain (CGA)

Recombinant Dog Glycoprotein hormones alpha chain (CGA) — Shared alpha chain of the active heterodimeric glycoprotein hormones thyrotropin/thyroid stimulating hormone/TSH, lutropin/luteinizing hormone/LH and follitropin/follicle stimulating hormone/FSH. Purity >90%.

SKU: BCRECP-002361 Category:
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Product Specifications

Product SkuBCRECP-002361
Product DescriptionRecombinant Dog Glycoprotein hormones alpha chain (CGA) Protein is expressed from E.coli with N-terminal 10xHis-tagged and C-terminal Myc-tagged. It contains 25-120aa. [Accession | Q9XSW8].
Uniprot No.Q9XSW8
Gene NamesCGA
PurityGreater than 90% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region25-120aa
SpeciesCanis lupus familiaris (Dog) (Canis familiaris)
Tag InfoN-terminal 10xHis-tagged and C-terminal Myc-tagged
Molecular weight18.1kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research Areasothers

Function

Shared alpha chain of the active heterodimeric glycoprotein hormones thyrotropin/thyroid stimulating hormone/TSH, lutropin/luteinizing hormone/LH and follitropin/follicle stimulating hormone/FSH. These hormones bind specific receptors on target cells that in turn activate downstream signaling pathways.

Biological Context

Subcellular Location: Secreted

Product Specifications

Recombinant Dog Glycoprotein hormones alpha chain (CGA) is a recombinant protein from Canis lupus familiaris (Dog) (Canis familiaris), expressed in E.coli, covering amino acids 25-120aa, with N-terminal 10xHis-tagged and C-terminal Myc-tagged tag, molecular weight 18.1kDa, purity Greater than 90% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

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Protein Biology

Function

Shared alpha chain of the active heterodimeric glycoprotein hormones thyrotropin/thyroid stimulating hormone/TSH, lutropin/luteinizing hormone/LH and follitropin/follicle stimulating hormone/FSH. These hormones bind specific receptors on target cells that in turn activate downstream signaling pathways

Subcellular Location

Secreted

Subunit

Heterodimer. The active hormones thyrotropin, lutropin and follitropin are heterodimers composed of CGA, a common alpha chain described here and a unique beta chain which confers their biological specificity to the hormones: TSHB for thyrotropin, LHB for lutropin and FSHB for follitropin

Gene: CGA  |  Organism: Canis lupus familiaris  |  Synonyms: Anterior pituitary glycoprotein hormones common subunit alpha; Follicle-stimulating hormone alpha chain; Follitropin alpha chain; Luteinizing hormone alpha chain; Lutropin alpha chain; Thyroid-stimulating hormone alpha chain; Thyrotropin alpha chain

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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