Recombinant Dog Parathyroid hormone/parathyroid hormone-related peptide receptor (PTH1R) Protein, Canis lupus familiaris (Dog) (Canis familiaris), in vitro E.coli expression system, N-terminal 10xHis-tagged, 65.0kDa, purity >85% as determined by SDS-PAGE.

Recombinant Dog Parathyroid hormone/parathyroid hormone-related peptide receptor (PTH1R) Protein

Recombinant Dog Parathyroid hormone/parathyroid hormone-related peptide receptor (PTH1R) Protein — G protein-coupled receptor for parathyroid hormone (PTH) and for parathyroid hormone-related peptide (PTHLH). Purity >85%.

SKU: BCRECP-00447TM Category:
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Product Specifications

Uniprot No.Q9TU31
Gene NamesPTH1R
PurityGreater than 85% as determined by SDS-PAGE.
Expression Systemin vitro E.coli expression system
Expression Region29-595aa
SpeciesCanis lupus familiaris (Dog) (Canis familiaris)
Tag InfoN-terminal 10xHis-tagged
Molecular weight65.0kDa
ActivityNot Test
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Function

G protein-coupled receptor for parathyroid hormone (PTH) and for parathyroid hormone-related peptide (PTHLH). Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of downstream effectors, such as adenylate cyclase (cAMP). PTH1R is coupled to G(s) G alpha proteins and mediates activation of adenylate cyclase activity.

Biological Context

Subcellular Location: Cell membrane (Multi-pass membrane protein)
Tissue Specificity: High levels in the kidney, with much lower levels in aorta, heart, lung, prostate, testis, and skeletal muscle

Product Specifications

Recombinant Dog Parathyroid hormone/parathyroid hormone-related peptide receptor (PTH1R) Protein is a recombinant protein from Canis lupus familiaris (Dog) (Canis familiaris), expressed in in vitro E.coli expression system, covering amino acids 29-595aa, with N-terminal 10xHis-tagged tag, molecular weight 65.0kDa, purity Greater than 85% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

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Protein Biology

Function

G protein-coupled receptor for parathyroid hormone (PTH) and for parathyroid hormone-related peptide (PTHLH) (PubMed:12153143). Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of downstream effectors, such as adenylate cyclase (cAMP) (By similarity). PTH1R is coupled to G(s) G alpha proteins and mediates activation of adenylate cyclase activity (By similarity). PTHLH dissociates from PTH1R more rapidly than PTH; as consequence, the cAMP response induced by PTHLH decays faster than the response induced by PTH (By similarity)

Subcellular Location

Cell membrane (Multi-pass membrane protein)

Tissue Specificity

High levels in the kidney, with much lower levels in aorta, heart, lung, prostate, testis, and skeletal muscle

Subunit

Homodimer in the absence of bound ligand. Peptide hormone binding leads to dissociation of the homodimer

Gene: PTH1R  |  Organism: Canis lupus familiaris  |  Synonyms: PTH/PTHrP type I receptor; Parathyroid hormone 1 receptor

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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