Recombinant DT3C (Diphtheria toxin & spg 3C domain) for Antibody Internalization Assay (Active) — recombinant protein from N/A, expressed in E.coli. Purity >90%.
| Product SKU | BCACP-000182 |
| Uniprot No. | P00588 P19909 |
| Gene Names | DT3C |
| Purity | Greater than 90% as determined by SDS-PAGE.Greater than 90% as determined by SEC-HPLC. |
| Expression System | E.coli |
| Species | N/A |
| Tag Info | N-terminal 6xHis-tagged |
| Molecular weight | 69.4 kDa |
| Activity | Measured by its binding ability in a functional ELISA. Immobilized Rat Mapt at 2 μg/ml can bind anti-MAPT recombinant antibody , the EC50 is 136.2-238.9 ng/mL. |
| Buffer | Lyophilized from a 0.2 μm filtered 20 mM Tris-HCl, 0.5 M NaCl, 6% Trehalose, pH 8.0 |
| Storage | Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles. |
Recombinant DT3C (Diphtheria toxin & spg 3C domain) for Antibody Internalization Assay (Active) is a recombinant protein from N/A, expressed in E.coli, with N-terminal 6xHis-tagged tag, molecular weight 69.4 kDa, purity Greater than 90% as determined by SDS-PAGE.Greater than 90% as determined by SEC-HPLC.. Suitable for ELISA and Western Blot applications.
Recommended applications depend on the target, product format, and product-specific validation data. Refer to the product datasheet or Certificate of Analysis (CoA), or contact BioCrest Sci for application guidance.
Biological Activity: Measured by its binding ability in a functional ELISA. Immobilized Rat Mapt at 2 μg/ml can bind anti-MAPT recombinant antibody , the EC50 is 136.2-238.9 ng/mL.
Storage: Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shipping & Lead Time: Availability, shipping conditions, and estimated lead time are confirmed in the formal quotation or order confirmation based on the product format, destination, and order requirements.
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Submit the Online Inquiry form with your required quantity and specifications, or email sales@biocrestsci.com. Available sizes, pricing, stock status, documentation, and estimated lead time are confirmed in the formal quotation.
Availability, dispatch timing, shipping conditions, and delivery estimates depend on the product format, destination, and order requirements. These details are confirmed in the formal quotation or order confirmation.
Follow the storage and handling instructions in the product-specific datasheet and Certificate of Analysis (CoA). Requirements may vary by product form and formulation; contact BioCrest Sci if product-specific guidance is needed.
Quality-control data such as identity, purity, endotoxin level, or biological activity are provided only when specified on this product page, the datasheet, or Certificate of Analysis (CoA). Biological activity is not implied unless explicitly stated.
