Recombinant Dugbe virus Nucleoprotein (N) — Binds dsRNA and ssRNA and probably participates in the packaging of viral genome. Purity >85%.
| Product Sku | BCRECP-003495 |
| Product Description | Recombinant Dugbe virus Nucleoprotein (N) Protein is expressed from E.coli with N-terminal 10xHis-tagged and C-terminal Myc-tagged. It contains 1-483aa. [Accession | Q8V336]. |
| Uniprot No. | Q8V336 |
| Gene Names | N |
| Purity | Greater than 85% as determined by SDS-PAGE. |
| Expression System | E.coli |
| Expression Region | 1-483aa |
| Species | Dugbe virus (isolate ArD44313) (DUGV) |
| Tag Info | N-terminal 10xHis-tagged and C-terminal Myc-tagged |
| Molecular weight | 61.4kDa |
| Activity | Please contact us to obtain bioactivity data. |
| Buffer | If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose. |
| Storage | Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles. |
| Research Areas | Microbiology |
Binds dsRNA and ssRNA and probably participates in the packaging of viral genome. In the dsRNA binding mode, the nucleocapsid protein specifically binds to the vRNA panhandle secondary structure formed at the termini of viral genome.
Recombinant Dugbe virus Nucleoprotein (N) — Binds dsRNA and ssRNA and probably participates in the packaging of viral genome. Purity >85%. Suitable for ELISA and Western Blot applications. Explore more Viral Antigen proteins →
SDS-PAGE: Single band at expected molecular weight confirming purity.
ELISA: Suitable as coating antigen or detection standard.
Western Blot: Compatible with standard Western Blot protocols.
Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.
Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.
Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.
Shelf Life: 12 months from date of receipt when stored as recommended.
Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.
Binds dsRNA and ssRNA and probably participates in the packaging of viral genome. In the dsRNA binding mode, the nucleocapsid protein specifically binds to the vRNA panhandle secondary structure formed at the termini of viral genome. Does not discriminate between viral and nonviral RNAs through ssRNA binding mode. Displays dsDNA endonuclease activity that is sequence non-specific
Virion
Probable homooligomer; forms a double superhelical polymer. Monomer
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Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.
Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.
Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.
