Recombinant Eisenia fetida Lysenin, Eisenia fetida (Red wiggler worm), E.coli, C-terminal 6xHis-tagged, 40.4kDa, purity >90% as determined by SDS-PAGE.

Recombinant Eisenia fetida Lysenin

Recombinant Eisenia fetida Lysenin — Pore-forming toxin that defensively acts against parasitic microorganisms by forming pores in sphingomyelin-containing membranes. Purity >90%.

SKU: BCRECP-003720 Category:
Online Inquiry →

Product Specifications

Product SkuBCRECP-003720
Product DescriptionRecombinant Eisenia fetida Lysenin Protein is expressed from E.coli with C-terminal 6xHis-tagged. It contains 1-297aa. [Accession | O18423].
Uniprot No.O18423
PurityGreater than 90% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region1-297aa
SpeciesEisenia fetida (Red wiggler worm)
Tag InfoC-terminal 6xHis-tagged
Molecular weight40.4kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasOthers

Function

Pore-forming toxin that defensively acts against parasitic microorganisms by forming pores in sphingomyelin-containing membranes. Has hemolytic activity and is also cytotoxic to spermatozoa of some species of invertebrates and many species of vertebrates and to amphibian larvae, guinea pig polymorphonuclear leukocytes, chicken fibroblasts, normal spleen cells and various tumor cells. Is lethal for various species of reptiles, amphibian, birds and mammals.

Product Specifications

Recombinant Eisenia fetida Lysenin is a recombinant protein, expressed in E.coli, covering amino acids 1-297aa, with C-terminal 6xHis-tagged tag, molecular weight 40.4kDa, purity Greater than 90% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

Related products

Protein Biology

Function

Pore-forming toxin that defensively acts against parasitic microorganisms by forming pores in sphingomyelin-containing membranes (PubMed:12676961, PubMed:9478988). Has hemolytic activity and is also cytotoxic to spermatozoa of some species of invertebrates and many species of vertebrates and to amphibian larvae, guinea pig polymorphonuclear leukocytes, chicken fibroblasts, normal spleen cells and various tumor cells (PubMed:10684578). Is lethal for various species of reptiles, amphibian, birds and mammals. Induces smooth muscle contraction (PubMed:9210594). It binds sphingomyelin and induces hemolysis in the same manner as lysenin-related protein 2, and is 10-fold more effective than lysenin-related protein 1 (PubMed:15274631)

Organism: Eisenia fetida

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

Shopping Cart
Scroll to Top