Recombinant Eisenia fetida Lysenin, Eisenia fetida (Red wiggler worm), E.coli, C-terminal 6xHis-tagged, 40.4kDa, purity >90% as determined by SDS-PAGE.

Recombinant Eisenia fetida Lysenin

Recombinant Eisenia fetida Lysenin — Pore-forming toxin that defensively acts against parasitic microorganisms by forming pores in sphingomyelin-containing membranes. Purity >90%.

SKU: BCRECP-003720 Category:
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Product Specifications

Product SkuBCRECP-003720
Product DescriptionRecombinant Eisenia fetida Lysenin Protein is expressed from E.coli with C-terminal 6xHis-tagged. It contains 1-297aa. [Accession | O18423].
Uniprot No.O18423
PurityGreater than 90% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region1-297aa
SpeciesEisenia fetida (Red wiggler worm)
Tag InfoC-terminal 6xHis-tagged
Molecular weight40.4kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasOthers

Function

Pore-forming toxin that defensively acts against parasitic microorganisms by forming pores in sphingomyelin-containing membranes. Has hemolytic activity and is also cytotoxic to spermatozoa of some species of invertebrates and many species of vertebrates and to amphibian larvae, guinea pig polymorphonuclear leukocytes, chicken fibroblasts, normal spleen cells and various tumor cells. Is lethal for various species of reptiles, amphibian, birds and mammals.

Product Specifications

Recombinant Eisenia fetida Lysenin is a recombinant protein, expressed in E.coli, covering amino acids 1-297aa, with C-terminal 6xHis-tagged tag, molecular weight 40.4kDa, purity Greater than 90% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

Recommended applications depend on the target, product format, and product-specific validation data. Refer to the product datasheet or Certificate of Analysis (CoA), or contact BioCrest Sci for application guidance.

Biological Activity: Please contact us to obtain bioactivity data.

Storage: Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shipping & Lead Time: Availability, shipping conditions, and estimated lead time are confirmed in the formal quotation or order confirmation based on the product format, destination, and order requirements.

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Protein Biology

Function

Pore-forming toxin that defensively acts against parasitic microorganisms by forming pores in sphingomyelin-containing membranes (PubMed:12676961, PubMed:9478988). Has hemolytic activity and is also cytotoxic to spermatozoa of some species of invertebrates and many species of vertebrates and to amphibian larvae, guinea pig polymorphonuclear leukocytes, chicken fibroblasts, normal spleen cells and various tumor cells (PubMed:10684578). Is lethal for various species of reptiles, amphibian, birds and mammals. Induces smooth muscle contraction (PubMed:9210594). It binds sphingomyelin and induces hemolysis in the same manner as lysenin-related protein 2, and is 10-fold more effective than lysenin-related protein 1 (PubMed:15274631)

Organism: Eisenia fetida

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Frequently Asked Questions

How do I order or inquire about this product?

Submit the Online Inquiry form with your required quantity and specifications, or email sales@biocrestsci.com. Available sizes, pricing, stock status, documentation, and estimated lead time are confirmed in the formal quotation.

What is the shipping and delivery time?

Availability, dispatch timing, shipping conditions, and delivery estimates depend on the product format, destination, and order requirements. These details are confirmed in the formal quotation or order confirmation.

How should this product be stored?

Follow the storage and handling instructions in the product-specific datasheet and Certificate of Analysis (CoA). Requirements may vary by product form and formulation; contact BioCrest Sci if product-specific guidance is needed.

What quality controls are performed on your products?

Quality-control data such as identity, purity, endotoxin level, or biological activity are provided only when specified on this product page, the datasheet, or Certificate of Analysis (CoA). Biological activity is not implied unless explicitly stated.

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