Recombinant Enterobacteria phage 933W Shiga-like toxin 2 subunit A (stxA2) (E189K), Enterobacteria phage 933W (Bacteriophage 933W), E.coli, N-terminal 10xHis-tagged and C-terminal Myc-tagged, 40.6kDa, purity >85% as determined by SDS-PAGE.

Recombinant Enterobacteria phage 933W Shiga-like toxin 2 subunit A (stxA2) (E189K)

Recombinant Enterobacteria phage 933W Shiga-like toxin 2 subunit A (stxA2) (E189K) — The A subunit is responsible for inhibiting protein synthesis through the catalytic inactivation of 60S ribosomal subunits. Purity >85%.

SKU: BCRECP-004501 Category:
Online Inquiry →

Product Specifications

Product SkuBCRECP-004501
Product DescriptionRecombinant Enterobacteria phage 933W Shiga-like toxin 2 subunit A (stxA2) (E189K) Protein is expressed from E.coli with N-terminal 10xHis-tagged and C-terminal Myc-tagged. It contains 23-319aa(E189K). [Accession | P09385].
Uniprot No.P09385
Gene NamesstxA2
PurityGreater than 85% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region23-319aa(E189K)
SpeciesEnterobacteria phage 933W (Bacteriophage 933W)
Tag InfoN-terminal 10xHis-tagged and C-terminal Myc-tagged
Molecular weight40.6kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasNeuroscience

Function

The A subunit is responsible for inhibiting protein synthesis through the catalytic inactivation of 60S ribosomal subunits. After endocytosis, the A subunit is cleaved by furin in two fragments, A1 and A2: A1 is the catalytically active fragment, and A2 is essential for holotoxin assembly with the B subunits.

Biological Context

Subcellular Location: Secreted

Product Specifications

Recombinant Enterobacteria phage 933W Shiga-like toxin 2 subunit A (stxA2) (E189K) is a recombinant protein from Enterobacteria phage 933W (Bacteriophage 933W), expressed in E.coli, covering amino acids 23-319aa(E189K), with N-terminal 10xHis-tagged and C-terminal Myc-tagged tag, molecular weight 40.6kDa, purity Greater than 85% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

Related products

Protein Biology

Function

The A subunit is responsible for inhibiting protein synthesis through the catalytic inactivation of 60S ribosomal subunits. After endocytosis, the A subunit is cleaved by furin in two fragments, A1 and A2: A1 is the catalytically active fragment, and A2 is essential for holotoxin assembly with the B subunits

Subcellular Location

Secreted

Subunit

Shiga-like toxin contains a single A subunit and multiple copies of a B subunit

Gene: stxA2  |  Organism: Escherichia phage 933W  |  Synonyms: Verocytotoxin 2 subunit A; Verotoxin 2 subunit A; rRNA N-glycosidase 2
Key Publications

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

Shopping Cart
Scroll to Top