Recombinant Enterobacteria phage K3 Holin (T), partial, Enterobacteria phage K3 (Bacteriophage K3), E.coli, N-terminal 10xHis-tagged and C-terminal Myc-tagged, 27.2kDa, purity >90% as determined by SDS-PAGE.

Recombinant Enterobacteria phage K3 Holin (T), partial

Recombinant Enterobacteria phage K3 Holin (T), partial — Accumulates harmlessly in the cytoplasmic membrane until it reaches a critical concentration that triggers the formation of micron-scale pores (holes) causing host cell membrane disruption and endo… Purity >90%.

SKU: BCRECP-004523 Category:
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Product Specifications

Product SkuBCRECP-004523
Product DescriptionRecombinant Enterobacteria phage K3 Holin (T) Protein is expressed from E.coli with N-terminal 10xHis-tagged and C-terminal Myc-tagged. It contains 50-218aa. [Accession | P10393].
Uniprot No.P10393
Gene NamesT
PurityGreater than 90% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region50-218aa
SpeciesEnterobacteria phage K3 (Bacteriophage K3)
Tag InfoN-terminal 10xHis-tagged and C-terminal Myc-tagged
Molecular weight27.2kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasOthers

Function

Accumulates harmlessly in the cytoplasmic membrane until it reaches a critical concentration that triggers the formation of micron-scale pores (holes) causing host cell membrane disruption and endolysin escape into the periplasmic space. Determines the precise timing of host cell lysis. Regulated by specific antiholins that somehow sense superinfections and then delay lysis.

Biological Context

Subcellular Location: Host cell inner membrane (Single-pass type II membrane protein)

Product Specifications

Recombinant Enterobacteria phage K3 Holin (T), partial is a recombinant protein from Enterobacteria phage K3 (Bacteriophage K3), expressed in E.coli, covering amino acids 50-218aa, with N-terminal 10xHis-tagged and C-terminal Myc-tagged tag, molecular weight 27.2kDa, purity Greater than 90% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

Recommended applications depend on the target, product format, and product-specific validation data. Refer to the product datasheet or Certificate of Analysis (CoA), or contact BioCrest Sci for application guidance.

Biological Activity: Please contact us to obtain bioactivity data.

Storage: Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shipping & Lead Time: Availability, shipping conditions, and estimated lead time are confirmed in the formal quotation or order confirmation based on the product format, destination, and order requirements.

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Protein Biology

Function

Accumulates harmlessly in the cytoplasmic membrane until it reaches a critical concentration that triggers the formation of micron-scale pores (holes) causing host cell membrane disruption and endolysin escape into the periplasmic space. Determines the precise timing of host cell lysis. Regulated by specific antiholins that somehow sense superinfections and then delay lysis. Participates with the endolysin and spanin proteins in the sequential events which lead to the programmed host cell lysis releasing the mature viral particles from the host cell

Subcellular Location

Host cell inner membrane (Single-pass type II membrane protein)

Subunit

Homomultimer. Heterotetramer composed of 2 holin and 2 antiholin. The holin-antiholin complex binds dsDNA. Interacts (via C-terminus) with antiholin (via C-terminus); this interaction blocks the holin homomultimerization and delays host cell lysis. Interacts (via N-terminus) with the lysis inhibition accessory protein rIII; this interaction stabilizes the holin-antiholin complex thereby resulting in a robust block of the hole formation

Gene: T  |  Organism: Enterobacteria phage K3  |  Synonyms: Lysis protein

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Submit the Online Inquiry form with your required quantity and specifications, or email sales@biocrestsci.com. Available sizes, pricing, stock status, documentation, and estimated lead time are confirmed in the formal quotation.

What is the shipping and delivery time?

Availability, dispatch timing, shipping conditions, and delivery estimates depend on the product format, destination, and order requirements. These details are confirmed in the formal quotation or order confirmation.

How should this product be stored?

Follow the storage and handling instructions in the product-specific datasheet and Certificate of Analysis (CoA). Requirements may vary by product form and formulation; contact BioCrest Sci if product-specific guidance is needed.

What quality controls are performed on your products?

Quality-control data such as identity, purity, endotoxin level, or biological activity are provided only when specified on this product page, the datasheet, or Certificate of Analysis (CoA). Biological activity is not implied unless explicitly stated.

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