Recombinant Enterobacteria phage RB69 DNA polymerase (43), partial, Escherichia phage RB69 (Bacteriophage RB69), E.coli, N-terminal 10xHis-tagged and C-terminal Myc-tagged, 35.4kDa, purity >90% as determined by SDS-PAGE.

Recombinant Enterobacteria phage RB69 DNA polymerase (43), partial

Recombinant Enterobacteria phage RB69 DNA polymerase (43), partial — Replicates the viral genomic DNA. Purity >90%.

SKU: BCRECP-001963 Category:
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Product Specifications

Product SkuBCRECP-001963
Product DescriptionRecombinant Enterobacteria phage RB69 DNA polymerase (43) Protein is expressed from E.coli with N-terminal 10xHis-tagged and C-terminal Myc-tagged. It contains 103-340aa. [Accession | Q38087].
Uniprot No.Q38087
Gene Names43
PurityGreater than 90% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region103-340aa
SpeciesEscherichia phage RB69 (Bacteriophage RB69)
Tag InfoN-terminal 10xHis-tagged and C-terminal Myc-tagged
Molecular weight35.4kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasOthers

Function

Replicates the viral genomic DNA. This polymerase possesses two enzymatic activities: DNA synthesis (polymerase) and an exonucleolytic activity that degrades single-stranded DNA in the 3'- to 5'-direction for proofreading purpose.

Product Specifications

Recombinant Enterobacteria phage RB69 DNA polymerase (43), partial is a recombinant protein from Escherichia phage RB69 (Bacteriophage RB69), expressed in E.coli, covering amino acids 103-340aa, with N-terminal 10xHis-tagged and C-terminal Myc-tagged tag, molecular weight 35.4kDa, purity Greater than 90% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

Recommended applications depend on the target, product format, and product-specific validation data. Refer to the product datasheet or Certificate of Analysis (CoA), or contact BioCrest Sci for application guidance.

Biological Activity: Please contact us to obtain bioactivity data.

Storage: Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shipping & Lead Time: Availability, shipping conditions, and estimated lead time are confirmed in the formal quotation or order confirmation based on the product format, destination, and order requirements.

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Protein Biology

Function

Replicates the viral genomic DNA. This polymerase possesses two enzymatic activities: DNA synthesis (polymerase) and an exonucleolytic activity that degrades single-stranded DNA in the 3'- to 5'-direction for proofreading purpose

Subunit

Part of the replicase complex that includes the DNA polymerase, the polymerase clamp, the clamp loader complex, the single-stranded DNA binding protein, and the primase/helicase (By similarity). Interacts with the polymerase clamp; this interaction constitutes the polymerase holoenzyme (PubMed:10535734)

Gene: 43  |  Organism: Escherichia phage RB69  |  Synonyms: Gp43
Key Publications

Explore closely related product collections, technology platforms, and custom production resources.

Frequently Asked Questions

How do I order or inquire about this product?

Submit the Online Inquiry form with your required quantity and specifications, or email sales@biocrestsci.com. Available sizes, pricing, stock status, documentation, and estimated lead time are confirmed in the formal quotation.

What is the shipping and delivery time?

Availability, dispatch timing, shipping conditions, and delivery estimates depend on the product format, destination, and order requirements. These details are confirmed in the formal quotation or order confirmation.

How should this product be stored?

Follow the storage and handling instructions in the product-specific datasheet and Certificate of Analysis (CoA). Requirements may vary by product form and formulation; contact BioCrest Sci if product-specific guidance is needed.

What quality controls are performed on your products?

Quality-control data such as identity, purity, endotoxin level, or biological activity are provided only when specified on this product page, the datasheet, or Certificate of Analysis (CoA). Biological activity is not implied unless explicitly stated.

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