Recombinant Enterobacteria phage RB69 DNA polymerase (43), partial, Escherichia phage RB69 (Bacteriophage RB69), E.coli, N-terminal 10xHis-tagged and C-terminal Myc-tagged, 35.4kDa, purity >90% as determined by SDS-PAGE.

Recombinant Enterobacteria phage RB69 DNA polymerase (43), partial

Recombinant Enterobacteria phage RB69 DNA polymerase (43), partial — Replicates the viral genomic DNA. Purity >90%.

SKU: BCRECP-001963 Category:
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Product Specifications

Product SkuBCRECP-001963
Product DescriptionRecombinant Enterobacteria phage RB69 DNA polymerase (43) Protein is expressed from E.coli with N-terminal 10xHis-tagged and C-terminal Myc-tagged. It contains 103-340aa. [Accession | Q38087].
Uniprot No.Q38087
Gene Names43
PurityGreater than 90% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region103-340aa
SpeciesEscherichia phage RB69 (Bacteriophage RB69)
Tag InfoN-terminal 10xHis-tagged and C-terminal Myc-tagged
Molecular weight35.4kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasOthers

Function

Replicates the viral genomic DNA. This polymerase possesses two enzymatic activities: DNA synthesis (polymerase) and an exonucleolytic activity that degrades single-stranded DNA in the 3'- to 5'-direction for proofreading purpose.

Product Specifications

Recombinant Enterobacteria phage RB69 DNA polymerase (43), partial is a recombinant protein from Escherichia phage RB69 (Bacteriophage RB69), expressed in E.coli, covering amino acids 103-340aa, with N-terminal 10xHis-tagged and C-terminal Myc-tagged tag, molecular weight 35.4kDa, purity Greater than 90% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

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Protein Biology

Function

Replicates the viral genomic DNA. This polymerase possesses two enzymatic activities: DNA synthesis (polymerase) and an exonucleolytic activity that degrades single-stranded DNA in the 3'- to 5'-direction for proofreading purpose

Subunit

Part of the replicase complex that includes the DNA polymerase, the polymerase clamp, the clamp loader complex, the single-stranded DNA binding protein, and the primase/helicase (By similarity). Interacts with the polymerase clamp; this interaction constitutes the polymerase holoenzyme (PubMed:10535734)

Gene: 43  |  Organism: Escherichia phage RB69  |  Synonyms: Gp43
Key Publications

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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