Recombinant Enterobacteria phage T4 ATP-dependent DNA helicase dda (dda), Enterobacteria phage T4 (Bacteriophage T4), E.coli, N-terminal 6xHis-tagged, 54.0kDa, purity >85% as determined by SDS-PAGE.

Recombinant Enterobacteria phage T4 ATP-dependent DNA helicase dda (dda)

Recombinant Enterobacteria phage T4 ATP-dependent DNA helicase dda (dda) — DNA helicase that stimulates viral DNA replication and recombination. Purity >85%.

SKU: BCRECP-002515 Category:
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Product Specifications

Product SkuBCRECP-002515
Product DescriptionRecombinant Enterobacteria phage T4 ATP-dependent DNA helicase dda (dda) Protein is expressed from E.coli with N-terminal 6xHis-tagged. It contains 1-439aa. [Accession | P32270].
Uniprot No.P32270
Gene Namesdda
PurityGreater than 85% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region1-439aa
SpeciesEnterobacteria phage T4 (Bacteriophage T4)
Tag InfoN-terminal 6xHis-tagged
Molecular weight54.0kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasNeuroscience

Function

DNA helicase that stimulates viral DNA replication and recombination. Plays a role in T4 DNA replication initiation by selecting and activating DNA origins. Acts by dissociating and reassociating with the DNA molecule being unwound.

Product Specifications

Recombinant Enterobacteria phage T4 ATP-dependent DNA helicase dda (dda) is a recombinant protein from Enterobacteria phage T4 (Bacteriophage T4), expressed in E.coli, covering amino acids 1-439aa, with N-terminal 6xHis-tagged tag, molecular weight 54.0kDa, purity Greater than 85% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

Recommended applications depend on the target, product format, and product-specific validation data. Refer to the product datasheet or Certificate of Analysis (CoA), or contact BioCrest Sci for application guidance.

Biological Activity: Please contact us to obtain bioactivity data.

Storage: Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shipping & Lead Time: Availability, shipping conditions, and estimated lead time are confirmed in the formal quotation or order confirmation based on the product format, destination, and order requirements.

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Protein Biology

Function

DNA helicase that stimulates viral DNA replication and recombination. Plays a role in T4 DNA replication initiation by selecting and activating DNA origins. Acts by dissociating and reassociating with the DNA molecule being unwound. Unwinds DNA as a monomer in a 5'-3' direction at a rate of 250 bp/s and can efficiently displace proteins from the DNA (PubMed:17823128, PubMed:22504228)

Subunit

Monomer (PubMed:12411580). Interacts with UvsX and gene 32 protein (PubMed:1328208). Interacts with the single-stranded DNA-binding protein; this interaction probably allows Dda to stimulate branch migration (PubMed:39417586)

Gene: dda  |  Organism: Enterobacteria phage T4  |  Synonyms: DNA 5'-3' helicase Dda
Key Publications

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How do I order or inquire about this product?

Submit the Online Inquiry form with your required quantity and specifications, or email sales@biocrestsci.com. Available sizes, pricing, stock status, documentation, and estimated lead time are confirmed in the formal quotation.

What is the shipping and delivery time?

Availability, dispatch timing, shipping conditions, and delivery estimates depend on the product format, destination, and order requirements. These details are confirmed in the formal quotation or order confirmation.

How should this product be stored?

Follow the storage and handling instructions in the product-specific datasheet and Certificate of Analysis (CoA). Requirements may vary by product form and formulation; contact BioCrest Sci if product-specific guidance is needed.

What quality controls are performed on your products?

Quality-control data such as identity, purity, endotoxin level, or biological activity are provided only when specified on this product page, the datasheet, or Certificate of Analysis (CoA). Biological activity is not implied unless explicitly stated.

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