Recombinant Enterobacteria phage T4 DNA alpha-glucosyltransferase (agt), Enterobacteria phage T4 (Bacteriophage T4), E.coli, N-terminal 10xHis-SUMO-tagged and C-terminal Myc-tagged, 62.8kDa, purity >85% as determined by SDS-PAGE.
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Recombinant Enterobacteria phage T4 DNA alpha-glucosyltransferase (agt)

Recombinant Enterobacteria phage T4 DNA alpha-glucosyltransferase (agt) — Catalyzes the transfer of glucose from uridine diphosphoglucose to 5-hydroxymethyl cytosine of T4 DNA to yield glucosyl 5-hydroxymethyl cytosine (glc-HMC). Purity >85%.

SKU: BCRECP-002039 Categories: ,
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Product Specifications

Product SkuBCRECP-002039
Product DescriptionRecombinant Enterobacteria phage T4 DNA alpha-glucosyltransferase (agt) Protein is expressed from E.coli with N-terminal 10xHis-SUMO-tagged and C-terminal Myc-tagged. It contains 1-400aa. [Accession | P04519].
Uniprot No.P04519
Gene Namesagt
PurityGreater than 85% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region1-400aa
SpeciesEnterobacteria phage T4 (Bacteriophage T4)
Tag InfoN-terminal 10xHis-SUMO-tagged and C-terminal Myc-tagged
Molecular weight62.8kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasOthers

Function

Catalyzes the transfer of glucose from uridine diphosphoglucose to 5-hydroxymethyl cytosine of T4 DNA to yield glucosyl 5-hydroxymethyl cytosine (glc-HMC). This DNA process seems to occur immediately after DNA synthesis since the DNA alpha-glucosyltransferase interacts with the clamp protein gp45. The glc-HMC modification protects the phage genome against its own nucleases and the host restriction endonuclease system.

Biological Context

Pathway: Genetic information processing; DNA modification

Product Specifications

Recombinant Enterobacteria phage T4 DNA alpha-glucosyltransferase (agt) is a recombinant protein from Enterobacteria phage T4 (Bacteriophage T4), expressed in E.coli, covering amino acids 1-400aa, with N-terminal 10xHis-SUMO-tagged and C-terminal Myc-tagged tag, molecular weight 62.8kDa, purity Greater than 85% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

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Protein Biology

Function

Catalyzes the transfer of glucose from uridine diphosphoglucose to 5-hydroxymethyl cytosine of T4 DNA to yield glucosyl 5-hydroxymethyl cytosine (glc-HMC) (PubMed:15381072). This DNA process seems to occur immediately after DNA synthesis since the DNA alpha-glucosyltransferase interacts with the clamp protein gp45 (PubMed:15381072). The glc-HMC modification protects the phage genome against its own nucleases and the host restriction endonuclease system (PubMed:15381072). The glc-HMC modification also protects against the host CRISPR-Cas9 defense system (PubMed:26081634)

Subunit

Interacts with clamp protein gp45

Pathway

Genetic information processing; DNA modification

Gene: agt  |  Organism: Enterobacteria phage T4
Key Publications

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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