Recombinant Enterobacteria phage T4 Single-stranded DNA-binding protein (32)

Function

Single-stranded DNA-binding protein that participates in viral DNA replication, recombination, and repair (Probable). Coats the lagging-strand ssDNA as the replication fork advances (PubMed:9079662). Stimulates the activities of viral DNA polymerase and DnaB-like SF4 replicative helicase, probably via its interaction with the helicase assembly factor (PubMed:14871889). Stimulates the unwinding activity of UvsW helicase, inhibits it DNA winding activity (PubMed:17092935). Together with DnaB-like SF4 replicative helicase and the helicase assembly factor, promotes pairing of two homologous DNA molecules containing complementary single-stranded regions and mediates homologous DNA strand exchange (PubMed:11459967). Also promotes the formation of joint molecules (PubMed:9079662). mRNA specific autogenous translational repressor (PubMed:15507125)

Subunit

Homodimer in the absence of DNA, monomer when binding DNA (PubMed:26275775). Interacts with the DNA helicase assembly protein; a ternary complex between the helicase assembly protein, the single-stranded DNA-binding protein and ssDNA is an obligatory intermediate in the helicase loading mechanism (PubMed:15507125, PubMed:24338568). Part of the replicase complex that includes the DNA polymerase, the polymerase clamp, the clamp loader complex, the single-stranded DNA binding protein, the primase, the DnaB-like SF4 replicative helicase and the helicase assembly factor (PubMed:16800624, PubMed:26102578). Interacts (via C-terminus) with the viral ATP-dependent DNA helicase Dda; this interaction probably allows Dda to stimulate branch migration (PubMed:25481875, PubMed:39417586). Interacts with the viral SF2 UvsW repair helicase (PubMed:23732982)

• Krisch H.M. et al. Nucleotide sequences involved in bacteriophage T4 gene 32 translational self-regulation. — Proc. Natl. Acad. Sci. U.S.A. (1982)
• Williams K.R. et al. Primary structure of the bacteriophage T4 DNA helix-destabilizing protein. — J. Biol. Chem. (1981)
• Miller E.S. et al. Bacteriophage T4 genome. — Microbiol. Mol. Biol. Rev. (2003)
• Pan Y.-C.E. et al. Amino acid sequence studies on T4 gene 32 DNA-binding proteins. — Hoppe-Seyler's Z. Physiol. Chem. (1980)
• Hurley J.M. et al. Assembly of the bacteriophage T4 replication machine requires the acidic carboxy terminus of gene 32 protein. — J. Mol. Biol. (1993)
• Giedroc D.P. et al. Zn(II) coordination domain mutants of T4 gene 32 protein. — Biochemistry (1992)
• Casas-Finet J.R. et al. Structural basis for the nucleic acid binding cooperativity of bacteriophage T4 gene 32 protein: the (Lys/Arg)3(Ser/Thr)2 (LAST) motif. — Proc. Natl. Acad. Sci. U.S.A. (1992)
• Barry J. et al. Purification and characterization of bacteriophage T4 gene 59 protein. A DNA helicase assembly protein involved in DNA replication. — J. Biol. Chem. (1994)