Recombinant Enterobacteria phage T4 Single-stranded DNA-binding protein (32), Enterobacteria phage T4 (Bacteriophage T4), E.coli, N-terminal 10xHis-tagged and C-terminal Myc-tagged, 41.8kDa, purity >90% as determined by SDS-PAGE.

Recombinant Enterobacteria phage T4 Single-stranded DNA-binding protein (32)

Recombinant Enterobacteria phage T4 Single-stranded DNA-binding protein (32) — Single-stranded DNA-binding protein that participates in viral DNA replication, recombination, and repair (Probable). Purity >90%.

SKU: BCRECP-001960 Category:
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Product Specifications

Product SkuBCRECP-001960
Product DescriptionRecombinant Enterobacteria phage T4 Single-stranded DNA-binding protein (32) Protein is expressed from E.coli with N-terminal 10xHis-tagged and C-terminal Myc-tagged. It contains 1-301aa. [Accession | P03695].
Uniprot No.P03695
Gene Names32
PurityGreater than 90% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region1-301aa
SpeciesEnterobacteria phage T4 (Bacteriophage T4)
Tag InfoN-terminal 10xHis-tagged and C-terminal Myc-tagged
Molecular weight41.8kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasOthers

Function

Single-stranded DNA-binding protein that participates in viral DNA replication, recombination, and repair (Probable). Coats the lagging-strand ssDNA as the replication fork advances. Stimulates the activities of viral DNA polymerase and DnaB-like SF4 replicative helicase, probably via its interaction with the helicase assembly factor.

Product Specifications

Recombinant Enterobacteria phage T4 Single-stranded DNA-binding protein (32) is a recombinant protein from Enterobacteria phage T4 (Bacteriophage T4), expressed in E.coli, covering amino acids 1-301aa, with N-terminal 10xHis-tagged and C-terminal Myc-tagged tag, molecular weight 41.8kDa, purity Greater than 90% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

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Protein Biology

Function

Single-stranded DNA-binding protein that participates in viral DNA replication, recombination, and repair (Probable). Coats the lagging-strand ssDNA as the replication fork advances (PubMed:9079662). Stimulates the activities of viral DNA polymerase and DnaB-like SF4 replicative helicase, probably via its interaction with the helicase assembly factor (PubMed:14871889). Stimulates the unwinding activity of UvsW helicase, inhibits it DNA winding activity (PubMed:17092935). Together with DnaB-like SF4 replicative helicase and the helicase assembly factor, promotes pairing of two homologous DNA molecules containing complementary single-stranded regions and mediates homologous DNA strand exchange (PubMed:11459967). Also promotes the formation of joint molecules (PubMed:9079662). mRNA specific autogenous translational repressor (PubMed:15507125)

Subunit

Homodimer in the absence of DNA, monomer when binding DNA (PubMed:26275775). Interacts with the DNA helicase assembly protein; a ternary complex between the helicase assembly protein, the single-stranded DNA-binding protein and ssDNA is an obligatory intermediate in the helicase loading mechanism (PubMed:15507125, PubMed:24338568). Part of the replicase complex that includes the DNA polymerase, the polymerase clamp, the clamp loader complex, the single-stranded DNA binding protein, the primase, the DnaB-like SF4 replicative helicase and the helicase assembly factor (PubMed:16800624, PubMed:26102578). Interacts (via C-terminus) with the viral ATP-dependent DNA helicase Dda; this interaction probably allows Dda to stimulate branch migration (PubMed:25481875, PubMed:39417586). Interacts with the viral SF2 UvsW repair helicase (PubMed:23732982)

Gene: 32  |  Organism: Enterobacteria phage T4  |  Synonyms: Gp32; Helix-destabilizing protein
Key Publications

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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