Recombinant Epstein-Barr virus Envelope glycoprotein H (gH), partial, Epstein-Barr virus (strain B95-8) (HHV-4) (Human herpesvirus 4), E.coli, N-terminal 6xHis-SUMO-tagged, 38.1kDa, purity >90% as determined by SDS-PAGE.
,

Recombinant Epstein-Barr virus Envelope glycoprotein H (gH), partial

Recombinant Epstein-Barr virus Envelope glycoprotein H (gH), partial — The heterodimer glycoprotein H-glycoprotein L is required for the fusion of viral and plasma membranes leading to virus entry into the host cell. Purity >90%.

SKU: BCRECP-002847 Categories: ,
Online Inquiry →

Product Specifications

Product SkuBCRECP-002847
Product DescriptionRecombinant Epstein-Barr virus Envelope glycoprotein H (gH) Protein is expressed from E.coli with N-terminal 6xHis-SUMO-tagged. It contains 19-218aa. [Accession | P03231].
Uniprot No.P03231
Gene NamesgH
PurityGreater than 90% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region19-218aa
SpeciesEpstein-Barr virus (strain B95-8) (HHV-4) (Human herpesvirus 4)
Tag InfoN-terminal 6xHis-SUMO-tagged
Molecular weight38.1kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasOthers

Function

The heterodimer glycoprotein H-glycoprotein L is required for the fusion of viral and plasma membranes leading to virus entry into the host cell. Following initial binding to host receptor, membrane fusion is mediated by the fusion machinery composed of gB and the heterodimer gH/gL. May also be involved in the fusion between the virion envelope and the outer nuclear membrane during virion morphogenesis.

Biological Context

Subcellular Location: Virion membrane (Single-pass type I membrane protein); Host cell membrane (Single-pass type I membrane protein); Host endosome membrane (Single-pass type I membrane protein)

Product Specifications

Recombinant Epstein-Barr virus Envelope glycoprotein H (gH), partial is a recombinant protein from Epstein-Barr virus (strain B95-8) (HHV-4) (Human herpesvirus 4), expressed in E.coli, covering amino acids 19-218aa, with N-terminal 6xHis-SUMO-tagged tag, molecular weight 38.1kDa, purity Greater than 90% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

Related products

Protein Biology

Function

The heterodimer glycoprotein H-glycoprotein L is required for the fusion of viral and plasma membranes leading to virus entry into the host cell. Following initial binding to host receptor, membrane fusion is mediated by the fusion machinery composed of gB and the heterodimer gH/gL. May also be involved in the fusion between the virion envelope and the outer nuclear membrane during virion morphogenesis. The heterodimer gH/gL targets also host EPHA2 to promote viral entry

Subcellular Location

Virion membrane (Single-pass type I membrane protein); Host cell membrane (Single-pass type I membrane protein); Host endosome membrane (Single-pass type I membrane protein)

Subunit

Interacts with glycoprotein L (gL); this interaction is necessary for the correct processing and cell surface expression of gH. The heterodimer gH/gL seems to interact with gB trimers during fusion. The heterodimer gH/gL interacts with host EPHA2 to facilitate virus internalization and fusion (PubMed:29292384). Interacts with glycoprotein 42/BZLF2 (PubMed:27929061)

Gene: gH  |  Organism: Epstein-Barr virus (strain B95-8)
Key Publications

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

Shopping Cart
Scroll to Top