![Recombinant Escherichia coli 3-oxoacyl-[acyl-carrier-protein] synthase 1 (fabB), Escherichia coli (strain K12), E.coli, N-terminal 6xHis-tagged and C-terminal Myc-tagged, 49.3kDa, purity >90% as determined by SDS-PAGE.](https://www.biocrestsci.com/wp-content/uploads/protein/1/BCRECP-002689_SDS.jpg)
Recombinant Escherichia coli 3-oxoacyl-[acyl-carrier-protein] synthase 1 (fabB) — Involved in the type II fatty acid elongation cycle. Purity >90%.
| Product Sku | BCRECP-002689 |
| Product Description | Recombinant Escherichia coli 3-oxoacyl-[acyl-carrier-protein] synthase 1 (fabB) Protein is expressed from E.coli with N-terminal 6xHis-tagged and C-terminal Myc-tagged. It contains 1-406aa. [Accession | P0A953]. |
| Uniprot No. | P0A953 |
| Gene Names | fabB |
| Purity | Greater than 90% as determined by SDS-PAGE. |
| Expression System | E.coli |
| Expression Region | 1-406aa |
| Species | Escherichia coli (strain K12) |
| Tag Info | N-terminal 6xHis-tagged and C-terminal Myc-tagged |
| Molecular weight | 49.3kDa |
| Activity | Please contact us to obtain bioactivity data. |
| Buffer | If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose. |
| Storage | Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles. |
| Research Areas | Others |
Involved in the type II fatty acid elongation cycle. Catalyzes the elongation of a wide range of acyl-ACP by the addition of two carbons from malonyl-ACP to an acyl acceptor.
Recombinant Escherichia coli 3-oxoacyl-[acyl-carrier-protein] synthase 1 (fabB) — Involved in the type II fatty acid elongation cycle. Purity >90%. Suitable for ELISA and Western Blot applications. Explore more Enzyme proteins →
Catalog number: BCRECP-002689.
SDS-PAGE: Single band at expected molecular weight confirming purity.
ELISA: Suitable as coating antigen or detection standard.
Western Blot: Compatible with standard Western Blot protocols.
Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.
Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.
Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.
Shelf Life: 12 months from date of receipt when stored as recommended.
Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.
Involved in the type II fatty acid elongation cycle. Catalyzes the elongation of a wide range of acyl-ACP by the addition of two carbons from malonyl-ACP to an acyl acceptor (PubMed:19679654, PubMed:22017312, PubMed:8910376, PubMed:9013860). Can also use unsaturated fatty acids (PubMed:19679654, PubMed:3076377, PubMed:8910376). Catalyzes a key reaction in unsaturated fatty acid (UFA) synthesis, the elongation of the cis-3-decenoyl-ACP produced by FabA (PubMed:19679654). Can use acyl chains from C-6 to C-14 (PubMed:19679654, PubMed:22017312, PubMed:8910376, PubMed:9013860). Has an absolute requirement for an ACP substrate as the acyl donor, and no activity is detected when both substrates are based on CoA (PubMed:22017312)
Cytoplasm
Homodimer
Lipid metabolism; fatty acid biosynthesis
Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.
Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.
Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.
Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.
