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Recombinant Escherichia coli 3-oxoacyl-[acyl-carrier-protein] synthase 1 (fabB)

Recombinant Escherichia coli 3-oxoacyl-[acyl-carrier-protein] synthase 1 (fabB) — Involved in the type II fatty acid elongation cycle. Purity >90%.

SKU: BCRECP-002689 Categories: ,

Product Specifications

Product SkuBCRECP-002689
Product DescriptionRecombinant Escherichia coli 3-oxoacyl-[acyl-carrier-protein] synthase 1 (fabB) Protein is expressed from E.coli with N-terminal 6xHis-tagged and C-terminal Myc-tagged. It contains 1-406aa. [Accession | P0A953].
Uniprot No.P0A953
Gene NamesfabB
PurityGreater than 90% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region1-406aa
SpeciesEscherichia coli (strain K12)
Tag InfoN-terminal 6xHis-tagged and C-terminal Myc-tagged
Molecular weight49.3kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasOthers

Function

Involved in the type II fatty acid elongation cycle. Catalyzes the elongation of a wide range of acyl-ACP by the addition of two carbons from malonyl-ACP to an acyl acceptor.

Product Specifications

Recombinant Escherichia coli 3-oxoacyl-[acyl-carrier-protein] synthase 1 (fabB) — Involved in the type II fatty acid elongation cycle. Purity >90%. Suitable for ELISA and Western Blot applications.

Catalog number: BCRECP-002689.

Recommended applications depend on the target, product format, and product-specific validation data. Refer to the product datasheet or Certificate of Analysis (CoA), or contact BioCrest Sci for application guidance.

Biological Activity: Please contact us to obtain bioactivity data.

Storage: Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shipping & Lead Time: Availability, shipping conditions, and estimated lead time are confirmed in the formal quotation or order confirmation based on the product format, destination, and order requirements.

Protein Biology

Function

Involved in the type II fatty acid elongation cycle. Catalyzes the elongation of a wide range of acyl-ACP by the addition of two carbons from malonyl-ACP to an acyl acceptor (PubMed:19679654, PubMed:22017312, PubMed:8910376, PubMed:9013860). Can also use unsaturated fatty acids (PubMed:19679654, PubMed:3076377, PubMed:8910376). Catalyzes a key reaction in unsaturated fatty acid (UFA) synthesis, the elongation of the cis-3-decenoyl-ACP produced by FabA (PubMed:19679654). Can use acyl chains from C-6 to C-14 (PubMed:19679654, PubMed:22017312, PubMed:8910376, PubMed:9013860). Has an absolute requirement for an ACP substrate as the acyl donor, and no activity is detected when both substrates are based on CoA (PubMed:22017312)

Subcellular Location

Cytoplasm

Subunit

Homodimer

Pathway

Lipid metabolism; fatty acid biosynthesis

Gene: fabB  |  Organism: Escherichia coli (strain K12)  |  Synonyms: 3-oxoacyl-[acyl-carrier-protein] synthase I; Beta-ketoacyl-ACP synthase I
Key Publications

Frequently Asked Questions

How do I order or inquire about this product?

Submit the Online Inquiry form with your required quantity and specifications, or email sales@biocrestsci.com. Available sizes, pricing, stock status, documentation, and estimated lead time are confirmed in the formal quotation.

What is the shipping and delivery time?

Availability, dispatch timing, shipping conditions, and delivery estimates depend on the product format, destination, and order requirements. These details are confirmed in the formal quotation or order confirmation.

How should this product be stored?

Follow the storage and handling instructions in the product-specific datasheet and Certificate of Analysis (CoA). Requirements may vary by product form and formulation; contact BioCrest Sci if product-specific guidance is needed.

What quality controls are performed on your products?

Quality-control data such as identity, purity, endotoxin level, or biological activity are provided only when specified on this product page, the datasheet, or Certificate of Analysis (CoA). Biological activity is not implied unless explicitly stated.

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