Recombinant Escherichia coli 5-methylthioadenosine/S-adenosylhomocysteine nucleosidase (mtnN), Escherichia coli (strain 55989 / EAEC), E.coli, N-terminal 10xHis-tagged and C-terminal Myc-tagged, 31.8kDa, purity >90% as determined by SDS-PAGE.

Recombinant Escherichia coli 5-methylthioadenosine/S-adenosylhomocysteine nucleosidase (mtnN)

Recombinant Escherichia coli 5-methylthioadenosine/S-adenosylhomocysteine nucleosidase (mtnN) — Catalyzes the irreversible cleavage of the glycosidic bond in both 5′-methylthioadenosine (MTA) and S-adenosylhomocysteine (SAH/AdoHcy) to adenine and the corresponding thioribose, 5′-methylthiorib…

SKU: BCRECP-003427 Category:
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Product Specifications

Product SkuBCRECP-003427
Product DescriptionRecombinant Escherichia coli 5-methylthioadenosine/S-adenosylhomocysteine nucleosidase (mtnN) Protein is expressed from E.coli with N-terminal 10xHis-tagged and C-terminal Myc-tagged. It contains 1-232aa. [Accession | B7LGM1].
Uniprot No.B7LGM1
Gene NamesmtnN
PurityGreater than 90% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region1-232aa
SpeciesEscherichia coli (strain 55989 / EAEC)
Tag InfoN-terminal 10xHis-tagged and C-terminal Myc-tagged
Molecular weight31.8kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasOthers

Function

Catalyzes the irreversible cleavage of the glycosidic bond in both 5'-methylthioadenosine (MTA) and S-adenosylhomocysteine (SAH/AdoHcy) to adenine and the corresponding thioribose, 5'-methylthioribose and S-ribosylhomocysteine, respectively. Also cleaves 5'-deoxyadenosine, a toxic by-product of radical S-adenosylmethionine (SAM) enzymes, into 5-deoxyribose and adenine.

Product Specifications

Recombinant Escherichia coli 5-methylthioadenosine/S-adenosylhomocysteine nucleosidase (mtnN) — Catalyzes the irreversible cleavage of the glycosidic bond in both 5'-methylthioadenosine (MTA) and S-adenosylhomocysteine (SAH/AdoHcy) to adenine and the corresponding thioribose, 5'-methylthiorib… Suitable for ELISA and Western Blot applications.

Recommended applications depend on the target, product format, and product-specific validation data. Refer to the product datasheet or Certificate of Analysis (CoA), or contact BioCrest Sci for application guidance.

Biological Activity: Please contact us to obtain bioactivity data.

Storage: Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shipping & Lead Time: Availability, shipping conditions, and estimated lead time are confirmed in the formal quotation or order confirmation based on the product format, destination, and order requirements.

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Protein Biology

Function

Catalyzes the irreversible cleavage of the glycosidic bond in both 5'-methylthioadenosine (MTA) and S-adenosylhomocysteine (SAH/AdoHcy) to adenine and the corresponding thioribose, 5'-methylthioribose and S-ribosylhomocysteine, respectively. Also cleaves 5'-deoxyadenosine, a toxic by-product of radical S-adenosylmethionine (SAM) enzymes, into 5-deoxyribose and adenine. Thus, is required for in vivo function of the radical SAM enzymes biotin synthase and lipoic acid synthase, that are inhibited by 5'-deoxyadenosine accumulation

Subunit

Homodimer

Pathway

Amino-acid biosynthesis; L-methionine biosynthesis via salvage pathway; S-methyl-5-thio-alpha-D-ribose 1-phosphate from S-methyl-5'-thioadenosine (hydrolase route): step 1/2

Gene: mtnN  |  Organism: Escherichia coli (strain 55989 / EAEC)  |  Synonyms: 5'-deoxyadenosine nucleosidase; 5'-methylthioadenosine nucleosidase; S-adenosylhomocysteine nucleosidase

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Quality-control data such as identity, purity, endotoxin level, or biological activity are provided only when specified on this product page, the datasheet, or Certificate of Analysis (CoA). Biological activity is not implied unless explicitly stated.

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